胚胎及新生期大鼠胃肠道胰岛淀粉样多肽与生长抑素的免疫组织化学定位

为探讨胚胎期及新生期大鼠胃肠道的胰岛淀粉样多肽（ＩＡＰＰ）与其他生物活性物质的关系，用免疫组织化学ＰＡＰ法，在相邻切片上分别显示第１５～２１ｄ大鼠胚胎及新生大鼠胃肠道的ＩＡＰＰ免疫反应性（ＩＲ）细胞和生长抑素（ＳＳ）ＩＲ细胞，观察了ＩＡＰＰ与ＳＳ在胚胎及新生大鼠胃肠道的定位分布。结果表明：第１５ｄ胚胎，胃肠道内未见ＩＡＰＰ－和ＳＳ－ＩＲ细胞。第１７ｄ胚胎，ＩＡＰＰ－与ＳＳ－ＩＲ细胞均很少，免疫染色较弱，着色较浅，免疫反应性细胞散布于分化未完全的肠上皮细胞间。胚胎１９ｄ，ＩＡＰＰ－和ＳＳ－ＩＲ细胞出现于胃和小肠，以十二指肠为多。胚胎第２１ｄ及新生期，胃肠道各段均可见到ＩＡＰＰ－和ＳＳ－ＩＲ细胞。经邻片比较证明，胚胎第１９ｄ始至新生期，十二指肠和空肠中部分ＩＡＰＰ－ＩＲ细胞与部分ＳＳ－ＩＲ细胞的定位相同，表明ＩＡＰＰ与ＳＳ在十二指肠和空肠Ｄ细胞中有共存。胃、回肠、结肠和直肠未见到ＩＡＰＰ与ＳＳ有细胞内共存的现象。本文对上述结果的意义进行了讨论

IMMUNOHISTOCHEMICAL LOCALIZATION OF IAPP AND SS IN THE GASTROINTESTINAL TRACT OF FETAL AND NEWBORN RATS

In order to explore the relationship between islet amyloid polypeptide (IAPP) and classical transmitters in the gastrointestinal tract of fetal and newborn rat, the distribution of IAPP immunoreactive (IR) cells and somatostatin (SS) IR cells were shown on serial adjacent sections separately by use of the immunohistochemical PAP method in this study. The coexistence of IAPP and SS in the gastrointestinal tract of fetal and newborn rat was examinaed. The result showed that no IAPP or SS IR cell was found in the gastrointestinal tract on the 15th day. On the 17th day, a few IAPP and SS IR cells could be observed in the differentiating intestinal epithelium. On the 19th day, IAPP and SS IR cells were found in the gastric and small intestinal epithelium and the cell number was more in the duodenum. The cells containing IAPP and SS IR were present in the whole gut from day 21 of fetal life to newborn. The co localization of IAPP and SS in some cells of duodenum and jejunum was proved as compared with adjacent sections. The results suggested that IAPP co existed with SS in D cells of the duodenum and jejunum epithelium. The co existence of IAPP and SS was not detected in the stomach, ilium, colon and rectum. The possible function on the co existence of IAPP and SS in the gastrointestinal tract of fetal and newborn rats was discussed in this paper.