| 4种常用蛋白质测定方法用于中药注射剂中大分子蛋白检测的适用性研究 |
| |
| 引用本文： | 王雪,张伟,李家春,黄文哲,王振中,萧伟.4种常用蛋白质测定方法用于中药注射剂中大分子蛋白检测的适用性研究[J].中草药,2015,46(15):2228-2231. |
| |
| 作者姓名： | 王雪 张伟 李家春 黄文哲 王振中 萧伟 |
| |
| 作者单位： | 江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001;江苏康缘药业股份有限公司, 江苏 连云港 222001;中药制药过程新技术国家重点实验室, 江苏 连云港 222001 |
| |
| 基金项目： | 国家重大新药创制项目(2013ZX09402203) |
| |
| 摘 要： | 目的探究4种常用蛋白质测定方法用于中药注射剂中大分子蛋白检测的适用性。方法比较磺基水杨酸法、考马斯亮蓝法、福林酚(Lowry)法、体积排阻色谱(SEC)法的灵敏度、专属性,并将4种方法分别用于测定5种市售中药注射剂中的大分子蛋白,比较测定结果。结果磺基水杨酸法灵敏度不高,考马斯亮蓝法和Lowry法的专属性较差,SEC法灵敏度高且专属性好。以SEC法测定5种市售中药注射剂样品,均未检出大分子蛋白。结论 SEC法可用于中药注射剂中大分子蛋白测定。
|
| 关 键 词： | 中药注射剂 蛋白质 考马斯亮蓝法 磺基水杨酸法 福林酚法 体积排阻色谱法 |
| 收稿时间： | 2/2/2015 12:00:00 AM |
Determinatoin of macromolecule protein in Chinese materia medica injection |
| |
| WANG Xue,ZHANG Wei,LI Jia-chun,HUANG Wen-zhe,WANG Zhen-zhong and XIAO Wei.Determinatoin of macromolecule protein in Chinese materia medica injection[J].Chinese Traditional and Herbal Drugs,2015,46(15):2228-2231. |
| |
| Authors: | WANG Xue ZHANG Wei LI Jia-chun HUANG Wen-zhe WANG Zhen-zhong and XIAO Wei |
| |
| Institution: | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China |
| |
| Abstract: | Objective To investigate the feasibility of four methods for macromolecule protein determination in Chinese materia medica (CMM) injection. Methods The sensitivity, specificity, and protein test results of sulfosalicylic acid, Coomassie brilliant blue, Lowry, and size exclusion chromatograph (SEC) methods on five kinds of CMM injections were compared. Results Sulfosalicylic acid method had low sensitivity, Coomassie brilliant blue and Lowry methods had poor specificities, and SEC method had high sensitivity and good specificity. No protein was detected in five kinds of CMM injection by SEC. Conclusion SEC is suitable for the determinatoin of macromolecule protein in CMM injection. |
| |
| Keywords: | Chinese materia medica injection protein Coomassie brilliant blue method sulfosalicylic acid method Lowry method size exclusion chromatograph method |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
| 点击此处可从《中草药》下载免费的PDF全文 |
|
