BALB/c小鼠肾缺血再灌注损伤模型的建立与评价

背景：对于一些药物研究,小鼠是理想的造模工具,但由于小鼠耐受性相对较差,肾脏及肾蒂小且难于寻找,容易增加实验误差,导致造模失败。目的：探讨BALB/c小鼠肾缺血再灌注损伤模型的建立方法,评价肾脏缺血时间对肾缺血再灌注损伤的影响。方法：采用微型动脉夹夹闭小鼠双侧肾蒂的方法建立雄性BALB/c小鼠肾缺血再灌注损伤模型,根据肾缺血时间不同分为0min组（对照组）、30min组、35min组、45min组,肾再灌注后24h观察肾功能和肾脏病理组织学的变化,比较不同的肾脏缺血时间对上述指标的影响;观察45min组小鼠肾缺血再灌注损伤后的生存率。结果与结论：模型成功率95.9%,与对照组相比,肾缺血30min组、35min组和45min组再灌注后24h血清肌酐、尿素氮和肾脏病理组织学评分均升高,肾缺血45min组生存率明显下降,差异均有显著性意义（P〈0.05）。结果提示,应用微型动脉夹夹闭小鼠双侧肾蒂的方法可制备稳定肾缺血再灌注损伤模型,雄性小鼠肾缺血35～45min是造模较为理想的肾缺血时间,所得模型效果满意。

Establishment and evaluation of renal ischemia/reperfusion injury models in BALB/c mice

BACKGROUND：For studies of some drugs,mouse serves as an ideal animal for model establishment. However,the failure rate is high due to poor toleration,small size of the kidney and renal pedicle. OBJECTIVE：To construct the model of renal ischemia/reperfusion injury （IRI） in BALB/c mice and evaluate influences of different ischemic time on IRI. METHODS：Renal arteries of mice were bilaterally clamped with micro-artery clamps to establish model of renal IRI in male BALB/c mice. According to different ischemic time,the mice were divided into four groups：0 （control）,30,35,and 45 minutes. The animals were sacrificed at 24 hours post-operatively. Renal function and pathology of the kidneys were examined. The situation of illness and survival in 45-minute group were observed. RESULTS AND CONCLUSION：The successful rate of model was 95.9%. Levels of serum creatinine and blood urea nitrogen as well as histological scores were remarkably increased in 30-,35-,and 45-minute groups compared with control group 24 hours after operation （P 0.05）. However,the survival rate was significantly lower in 45-minute group （P 0.05）. Results show that stable model of renal IRI can be gained in BALB/c mice by applying micro-artery clamp to incarcerate bilateral renal arteries. The optimal renal ischemia time is 35-45 minutes in male mice.