前蛙跳式自体预贮血在猪心脏体外循环手术模型中的应用（英文）

背景：同种异体输血在相同血型的人类中已经得到实现,但关于猪的血型类型及如何异体输血国内外无确切报道。目的：探讨术前蛙跳式自体预贮血在猪心脏体外循环手术模型中应用的可行性。方法：16只家猪随机区组法分为2组,自体预贮血组、同种异体输血组。另4头猪放血供同种异体输血组异体输血应用。两组猪均在体外循环下行心脏手术。自体预贮血组手术全程仅输自体血,同种异体输血组手术全程输注等量异体血。记录自体预贮血组放血前后的血红蛋白及血细胞压积,两组术前基础值、体外循环过程中、体外循环结束后及术后1d的血红蛋白值。结果与结论：20只实验猪全身血容量（2500±428）mL;自体预贮血组第1次预计放血量为（501±86）mL,实际放血量为（493±93）mL;第2次预计放血量为（750±128）mL,实际放血量为（719±98）mL。自体预贮血组猪采血前后血红蛋白计数及血细胞压积差异有显著性意义（P〈0.01）。自体预贮血组术后1d时点血红蛋白数值明显高于同种异体输血组（P〈0.01）,两组其余术前基础值、体外循环过程中、体外循环结束后时点血红蛋白数值差异无显著性意义（P〉0.05）;与术前比较,两组术后1d时点血红蛋白值显著低于术前基础值（P〈0.01）。自体预贮血组存活率明显高于同种异体输血组（P〈0.01）。结果说明与同种异体输血相比,术前蛙跳式自体预贮血安全有效,术后猪成活率更高。

Preoperative autologous blood donation using the leap-frog technique for cardiopulmonary bypass surgery in a swine model

BACKGROUND：Allogeneic blood transfusion in humans of the same blood type has been implemented,but studies regarding swine blood type and how to perform allogeneic blood transfusion have been rarely reported. OBJECTIVE：To investigate the feasibility of preoperative autologous blood donation using the leap-frog technique for cardiopulmonary bypass surgery in a swine model. METHODS：Sixteen domestic swine were randomly divided into two groups：autologous blood donation and allogeneic blood transfusion. Another four swine were used as blood donors. Two groups of swine underwent cardiopulmonary bypass surgery. The autologous blood donation group received self-transfusion reserved before surgery while the allogeneic blood transfusion group received the same amount of allogeneic blood. Hemoglobin （Hb） concentration and hematocrit （Hct） level prior to and after donation in the autologous blood donation group was recorded. Hb concentration in the two groups was recorded prior to,during,immediately after,and 1 day after the surgery. RESULTS AND CONCLUSION：The total blood volume of each experimental swine was （2500±428） mL. For the autologous blood donation group,the predicted blood volume of the first donation was （501±86） mL and the actual blood volume was （493±93） mL; in the second donation,the predicted blood volume was （750±128） mL and the actual blood volume was （719±98） mL. There was a significant difference in Hb concentration and Hct level between prior to and after donation in the autologous blood donation group （P 0.01）. Hb concentration at 1 day after the surgery was significantly higher in the autologous blood donation group than in the allogeneic blood transfusion group （P 0.01）,while no significant difference in Hb concentration existed between these two groups prior to,during,and immediately after the surgery （P 0.05）. Compared with prior to surgery,Hb concentration in each group was significantly lower at 1 day after the surgery （P 0.01）. The autologous blood donation group exhibited significantly higher survival rate of swine than the allogeneic blood transfusion group （P 0.01）. These findings indicate that compared with allogeneic blood transfusion,preoperative autologous blood donation using the leap-frog technique appears to be a safe,effective method with a high survival rate for cardiopulmonary bypass surgery.