周围神经损伤修复与再生中的基因治疗

背景：周围神经损伤后功能恢复不够理想。基因治疗为其功能修复提供了新的方法。目的：从功能基因、转染载体选择、基因翻译因子生物学效应3方面进行综述,为周围神经损伤的基因治疗研究提供参考。方法：由第一作者应用计算机检索PubMed和中国期刊全文数据库（CNKI）2006/2010相关文献。在标题、摘要、关键词中以＂peripheral nerve injury,gene therapy,virus vector＂或＂周围神经损伤、基因治疗、病毒载体＂为检索词进行检索。选择与周围神经损伤的基因治疗有关的文献,同一领域文献则选择近期发表及发表在权威杂志的文章。结果与结论：共检索到35篇文章,按纳入和排除标准对文献进行筛选,保留20篇文章进行综述。目前,周围神经损伤的基因治疗技术已经日趋成熟,如转基因的腺病毒表达的骨形态发生蛋白7和Ad-32Ep65-Flag基因等,有望成为临床修复周围神经损伤的重要手段。

Gene therapy in the repair and regeneration of peripheral nerve injury

BACKGROUND：Functional recovery of peripheral nerve after repair is not ideal.Gene therapy provides a new method for repair peripheral nerve injury.OBJECTIVE：The paper reviewed from three areas,including functional gene,transfer vector selection,and biological effects of gene translation factors,aimed to provide reference for gene therapy for peripheral nerve injury.METHODS：A computer-based online search of PubMed and CNKI between 2006 and 2010 was performed to search related articles with the key words of ＂peripheral nerve injury,gene therapy,virus vector＂ both in English and Chinese.Literatures related to gene therapy for peripheral nerve injury were selected,in the same field,the articles published lately or published by authoritative journals were preferred. RESULTS AND CONCLUSION：A total of 35 articles were searched by computer and 21 documents were included after screening.Currently,technology of gene therapy for peripheral nerve injury has matured,such as transgenic adenovirus bone morphogenetic protein 7 and Ad-32Ep65-Flag gene,the clinical repair of peripheral nerve is expected to become an important means.