二元镁合金在细胞培养基中的耐腐蚀能力及其生物相容性

背景：尽管已经有很多研究报道镁及其合金具有一定的骨传导性和可降解性,可以做为承重骨科内植物材料,但是还需要做更多的工作来正确评估它的潜力所在。目的：检测镁和二元镁合金在细胞培养基中的降解特性和对成人骨髓间充质干细胞活性的影响。方法：制备纯镁和8种二元镁合金（Mg-Al、Mg-Ca、Mg-Mn、Mg-Sn、Mg-Si、Mg-Y、Mg-Zn、Mg-Zr）样品;采用α-MEM细胞培养基（含体积分数为10%胎牛血清）模拟体内环境下制备标准浸提液,并检测pH值;电感耦合等离子体原子发射光谱仪检测浸提液中镁和添加元素含量;AlamarBlue法检测培养达1,3,5,7d时100%、50%、25%浓度的纯镁和8种镁合金浸提液对成人骨髓间充质干细胞增殖的影响,判断8种二元镁合金的生物相容性。结果与结论：Mg-Ca、Mg-Y合金耐腐蚀性较差,浸提液中Mg2＋质量浓度分别达到（408.0±37.9）mg/L和（351±15.3）mg/L,pH值分别为8.87±0.19和8.84±0.15;其次是Mg-Zr合金;其他二元合金耐腐蚀性与纯镁相当。结果表明纯镁和8种二元镁合金100%含量的浸提液均对成人骨髓间充质干细胞的增殖有显著抑制作用,当Mg2＋质量浓度低于110mg/L,pH值7.35~7.65时,纯镁和8种二元镁合金对成人骨髓间充质干细胞的增殖无显著影响。

Corrosion resistance and biocompatibility of binary magnesium alloys in cell culture medium

BACKGROUND：Degrading magnesium and magnesium alloys are a new class of implant materials suitable for orthopedic surgery due to its bone conductibility and degradability,so further research on the evaluation of magnesium is very necessary.OBJECTIVE：To determine the degradability of magnesium and binary magnesium alloys in cell culture medium and their influence on the viability of adult human bone marrow mesenchymal stem cells.METHODS：Eight alloying elements Al,Ca,Mn,Si,Sn,Y,Zn and Zr were added into magnesium individually to fabricate binary Mg-1X（wt.%） alloys.Pure magnesium and eight binary magnesium alloys were immersed in α-MEM（containing 10% fetal bovine serum） to prepare extracts.The pH values of the extraction media were measured.Magnesium and alloy element ions in the extraction medium were analyzed using inductively coupled plasma-atomic emission spectrometry.Adult human bone marrow mesenchymal stem cells were cultured in extracts of 100%,50%,25% magnesium and eight binary magnesium alloys for 1,3,5,7 days.The indirect effects of magnesium alloys on viability of adult human bone marrow mesenchymal stem cells were detected using Alamar Blue？ cell viability reagent.RESULTS AND CONCLUSION：Mg-Ca and Mg-Y alloys showed poor corrosion resistance.The Mg2＋ concentration in liquid was（408.0±37.9） mg/L and（351±15.3） mg/L,respectively,while pH value was 8.87±0.19 and 8.84±0.15.Next is Mg-Zr alloy;other binary alloys were equal to pure Mg regarding corrosion resistance.The magnesium and eight binary magnesium alloys extracts at 100% concentration significantly inhibited the viability of adult human bone marrow mesenchymal stem cells.But the extracts did not inhibit the cell viability while Mg2＋ concentration was ≤ 110 mg/L and pH value of 7.35-7.65.