胚胎干细胞及在生殖医学中的应用

背景：有报道胚胎干细胞体外培养可分化为原始生殖细胞并进一步生成配子,受精后发育为胚泡,并产生成活的子代。目的：阐述近年胚胎干细胞在生殖医学中的研究进展。方法：应用计算机检索清华同方系列数据库2000/2009有关生殖细胞、中药的相关研究文章,检索词：生殖细胞、中药,并限定文章语言种类为中文;同时应用计算机检索Pubmed数据库1998/2009相关文章,检索词：embryonic stem cells,stem cells,germ cells,oocyte,sperm,differentiation,限定文章语言种类为＂English＂。结果与结论：胚胎干细胞在体外分化受到内、外源因素的影响,可以诱导分化为精子和卵母细胞,是研究哺乳动物早期胚胎发育的理想模型;目前研究体外培养原始生殖细胞和配子是可行的,但体外培养生殖细胞的特性和功能仍不确定,要应用于临床需进一步确定其安全性和有效性。

Application of embryonic stem cells to reproductive medicine

BACKGROUND：It has been reported that embryonic stem cells can differentiate into primordial germ cells in vitro culture and to further generate gametes then develop into blastocysts after fertilization,and produce live filial generation. OBJECTIVE：To expound the research progress of embryonic stem cells in reproductive medicine in recent years. METHODS：Tongfang database system was used to search for research articles published from 2000 to 2009 on germ cells and Chinese medicine related to the key words ＂germ cells,medicine＂ ,and the language was limited to Chinese; the same searching PubMed database articles published from 1998 to 2009 with the key words of ＂embryonic stem cells,stem cells,germ cells,oocyte,sperm,differentiation＂ ,and the language was limited to English. RESULTS AND CONCLUSION：Embryonic stem cells in vitro differentiation affected by internal and external factors,can be induced to differentiate into sperm and oocytes,is an ideal model on the study of early embryonic development in mammals. At present,it is feasible to in vitro culture primordial germ cells and gamete,but the characteristics and function of in vitro cultured germ cells remain unclear and it is necessary to further identify its safety and efficacy in clinic.