干细胞应力学效应机制的研究与进展

背景：近年来多种细胞信号转导的分子基础与相关功能调控机制的研究已取得重要进展,但干细胞的生物学行为相对复杂,其增殖分化的相关信号转导途径和机制尚未明确。目的：复习相关文献,就干细胞力学影响下相关信号通路的研究现状与应用前景进行综述。方法：应用计算机检索CNKI和PubMed数据库中2005/2010关于＂干细胞应力学效应机制＂的文章,以＂干细胞,信号转导＂或＂干细胞,应力＂为检索词进行检索。选择文章内容与干细胞信号转导通路相关,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到中英文共179篇文献,根据纳入标准选择33篇文章进行综述。结果与结论：调节干细胞应力下增殖与分化的多条信号通路,包括F-actin、Ca2＋、MAPK、Wnt、Notch等,在干细胞的增殖分化中发挥着重要调控作用。应力作用下细胞内力学信号转导和基因表达调控的确切机制将是今后干细胞研究领域的重要内容。

Development of mechanical mechanisms of stem cells

BACKGROUND：Recently,studies addressing molecular basis of various cell signal transduction and regulation mechanism of relevant functions have obtained important progress,but the biological behavior of stem cells is complicated. The related signal transduction pathway and mechanism of proliferation and differentiation remain unclear. OBJECTIVE：To review relevant literatures,and to summarize the present status and application prospect of stem cell related signal pathway affected by cytomechanics. METHODS：China National Knowledge Infrastructure and PubMed database were retrieved by computer for articles concerning mechanical effect of mechanisms for stem cells published from 2005 to 2010. The key words were ＂stem cell,signal transduction＂ or ＂stem cell,stress＂. The articles on signal transduction pathway of stem cells published recently in the same field or published in the authorized journals were selected,and totally 179 articles were primarily selected. Finally,33 articles were included in accordance with inclusion criteria. RESULTS AND CONCLUSION：Many different signal pathways had been involved in the proliferation and differentiation of stem cells. The stem cells could play an important role in the stem appreciation and differentiation which were modulated by several different signal pathways,including F-actin,Ca2＋,MAPK,Wnt and Notch. The exact mechanism of mechanics signal transduction and gene expression under stress condition will be an important content in future stem cell research.