超顺磁性氧化铁标记的骨髓间充质干细胞

背景：目前,超顺磁性氧化铁纳米颗粒标记的骨髓间充质干细胞在治疗心、肝、肾及中枢神经系统疾病方面已经得到了快速的应用。目的：对国内外应用超顺磁性氧化铁标记骨髓间充质干细胞的现状及新进展作一综述。方法：应用计算机检索CNKI和Pubmed数据库中2001-12/2009-12关于超顺磁性氧化铁标记骨髓间充质干细胞的文章,在标题和摘要中以＂超顺磁性氧化铁,干细胞,标记,磁共振成像＂或＂superparamagnetic iron oxide,stem cells,label,magnetic resonance imaging＂为检索词进行检索。选择近5年内文章内容与超顺磁性氧化铁标记骨髓间充质干细胞有关者,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到243篇文献,根据纳入标准选择关于超顺磁性氧化铁标记骨髓间充质干细胞的40篇文献进行综述。结果与结论：以往通过光镜识别干细胞标记物的研究中,必须用传统的组织病理学检查,从体内取得组织才能进行细胞探测,这显然不适合进行动态观察,更不适用于临床研究。超顺磁性氧化铁可以有效地进行活细胞标记,而这种标记方法不会影响细胞的活力、生长、分裂、迁移、分化等生物学功能,对细胞没有毒性,具有良好的安全性,可以用MRI进行活体示踪研究。

Bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide

BACKGROUND：At present,superparamagnetic iron oxide labeled bone marrow mesenchynal stem cells （BMSCs） have been rapidly applied in the treatment of heart,liver,kidney and central nervous system disease. OBJECTIVE：To summarize the present situation and progress about superparamagnetic iron oxide labeled BMSCs at home and abroad. METHODS：The database of CNKI and PubMed （2001-12/2009-12） was used to search the related articles about superparamagnetic iron oxide labeled BMSCs.Key words were ＂superparamagnetic iron oxide,stem cells,label,magnetic resonance imaging＂ that were searched in title and abstract. The articles related to superparamagnetic iron oxide labeled BMSCs published in recently five years,and for articles in the same field,those published recently or in authorized journals were selected. There were 243 articles after the initial survey. A total of 40 articles related to superparamagnetic iron oxide labeled BMSCs were included according to inclusion criteria for this review. RESULTS AND CONCLUSION：In previous studies addressing stem cell markers identified by light microscope,traditional histopathological examination was utilized,and the tissues were obtained in vivo. These are not suitable for dynamic observation,and not fit for clinical study. Superparamagnetic iron oxide can be used to effectively label living cells. This method cannot affect cell viability,growth,division,migration and differentiation,does not have cytotoxicity,with good safety,and magnetic resonance imaging can be utilized for in vivo tracer study.