高频脉冲电磁场对骨髓间充质干细胞增殖和成骨分化的影响

背景：采用低频脉冲电磁场干预骨髓间充质干细胞增殖分化的研究很多,但采用高频（〉300MHz）脉冲电磁场干预的研究国内未见报道。目的：观察〉300MHz高频脉冲电磁场照射能否促进骨髓间充质干细胞增殖,并向成骨分化。方法：分离培养SD大鼠骨髓间充质干细胞,取第3代细胞随机分为4组：成骨诱导组、成骨诱导＋电磁照射组、电磁场照射组、空白对照组。观察各组骨髓间充质干细胞培养过程中细胞形态、数量、总蛋白量等方面变化。结果与结论：与未经高频脉冲电磁场照射组相比,经高频脉冲电磁场照射后,骨髓间充质干细胞胞体稍有增多,但分化方面区别微弱。电磁场照射组细胞增殖速度、总蛋白含量均低于较空白对照组（P〈0.05）。但电磁照射组细胞凋亡率较空白对照组增加（P〈0.05）。说明高频脉冲电磁场促进骨髓间充质干细胞的成骨分化趋势不明显,可抑制其增殖,促进其凋亡。

Effects of high-frequency pulsed electromagnetic fields on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

BACKGROUND：The researches about low-frequency pulsed electromagnetic fields（PEMFs） interfering with bone marrow mesenchymal stem cells（BMSCs） proliferation and differentiation are many,but there is no report concerning high-frequency（ 300 MHz） PEMFs.OBJECTIVE：To explore whether high-frequency（ 300 MHz） PEMFs can promote proliferation and osteogenic differentiation of BMSCs.METHODS：BMSCs were isolated from Sprague-Dawley rats.The third passage of BMSCs were obtained and divided into four groups：osteogenic induction group,osteogenic induction＋PEMF irradiation group,PEMF irradiation group and blank control group.Changes in morphology,number and amount of total protein were observed during BMSC culture in each group.RESULTS AND CONCLUSION：The number of BMSC bodies was increased,but the differentiation ability was weak following PEMF irradiation.The speed of cell proliferation and content of total protein were lower in the PEMF irradiation group compared with blank control group（P 0.05）.However,cell apoptotic rate was greater in the PEMF irradiation group compared with blank control group（P 0.05）.Results indicated that promoting effects of high-frequency PEMFs on osteogenic differentiation of BMSCs are not obvious.High-frequency PEMFs can suppress BMSC proliferation and contribute to BMSC apoptosis.