亚甲蓝/光化学法灭活病毒对血浆成分的影响

背景：对血液进行病毒灭活是保障安全输血的措施之一,亚甲蓝/光化学法灭活人血浆中病毒的效果已被证实,但其对血浆成分影响的报道很少。目的：观察亚甲蓝光化学法病毒灭活血浆对血液成分结构和功能的影响。方法：随机选择40份采血后6h内400mL全血制备的新鲜血浆称质量留样,然后与亚甲蓝病毒灭活过滤器无菌连接,亚甲蓝的终浓度在0.9～1.3μmol/L。将加入亚甲蓝的血浆置入4℃病毒灭活箱的搁架上,摆动频率60次/min,利用32000～38000Lx光照强度的可见光4℃照射35min,将光照后的血浆通过病毒灭活过滤器滤除亚甲蓝和残余白细胞,混匀后留样10mL,立即置于-80℃冰箱冻存。检测照射前后样品的血浆量、亚甲蓝浓度、FⅧ∶C、FⅤ∶C、VWF、Fib含量的变化。结果与结论：血浆病毒灭活后血浆容量、FⅧ∶C、FⅤ∶C、VWF、Fib的回收率分别为（96.39±1.73）%、（82.55±9.25）%、（81.03±15.27）%、（93.25±6.17）%、（81.61±14.25）%。亚甲蓝光化学法灭活血浆病毒对血浆中大多数成分的影响不明显,可以满足临床安全输血的需求。

Effects of methylene blue photochemical virus inactivation on plasma components

BACKGROUND：Virus inactivation to blood is one of blood transfusion safety measures. The effect of virus inactivation on human plasma by methylene blue/light has been confirmed,but the report concerning virus inactivation to plasma on plasma components is very few. OBJECTIVE：To observe the effects of methylene blue photochemical virus inactivation to blood plasma on blood components＇ structure and function. METHODS：A total of 40 blood samples were randomly selected. Fresh plasma from 400 mL whole blood within 6 hours was prepared and the quality was weighed for reserve samples,and then the filter with methylene blue virus inactivation was sterilely connected. The final concentration of methylene blue was 0.9-1.3 μmol/L. Plasma added to methylene blue virus inactivation into 4 ℃ was put on a shelf box,with beat frequency of 60 times/minutes. 32 000-38 000 Lx intensity of 4 ℃ visible light was used to irradiate for 35 minutes. The residual methylene blue and white blood cells were filtered out from the plasma after illumination by the virus inactivated filters,mixed and remained sample 10 mL,and immediately placed in -80 ℃ refrigerator. The amount of plasma samples before and after exposure,methylene blue concentration,F Ⅷ：C,F Ⅴ：C,VWF,Fib Content was detected. RESULTS AND CONCLUSION：After virus inactivated plasma,the recovery rate of plasma volume,F Ⅷ：C,F Ⅴ：C,VWF,Fib were （96.39 ± 1.73）%,（82.55 ± 9.25）%,（81.03 ± 15.27）%,（ 93.25 ± 6.17）%,（81.61 ± 14.25）%. The effect of methylene blue photochemical virus inactivation in plasma on most components of the plasma is not obvious,and meets the clinical demand for safe blood transfusion.