骨髓间充质干细胞对慢性哮喘小鼠血清白细胞介素12和白细胞介素4水平的影响

背景：研究显示,骨髓间充质干细胞移植通过减轻炎症程度来改善急性肺损伤的病情,而骨髓间充质干细胞移植对哮喘疾病的研究甚少。目的：观察慢性哮喘小鼠移植同种异体骨髓间充质干细胞后,其血清白细胞介素12和白细胞介素4水平的变化。方法：取40只雌性BALB/c小鼠,随机分为4组,正常对照组和骨髓间充质干细胞对照组以PBS致敏及激发小鼠,于第21天激发前气管内注射PBS或骨髓间充质干细胞30μL。哮喘模型组和骨髓间充质干细胞治疗组用鸡卵白蛋白制备慢性哮喘模型,于第21天激发前气管内注射PBS或骨髓间充质干细胞30μL。采用ELISA法检测各组小鼠血清炎症因子水平。结果与结论：病理提示哮喘模型组支气管上皮黏膜脱落,同时上皮黏膜有杯状细胞增生,部分管腔内大量黏液栓塞;气道、血管旁有大量炎性细胞浸润以及气道平滑肌细胞增生和肥大。正常对照组及骨髓间充质干细胞对照组无气道炎症及气道重塑的表现,而骨髓间充质干细胞治疗组气道炎症及气道重塑明显减轻。对比骨髓间充质干细胞对照组及正常对照组,哮喘模型组白细胞介素12明显减低,白细胞介素13和白细胞介素4明显增高;而骨髓间充质干细胞治疗组较哮喘模型组白细胞介素12明显升高,白细胞介素4明显降低。结果表明骨髓间充质干细胞治疗哮喘可能通过降低白细胞介素4水平,提高体内白细胞介素12水平,从而改善气道炎症及气道重塑的程度。

Effects of bone marrow mesenchymal stem cells on the serum levels of interleukin-4 and interleukin-12 in chronic asthmatic mice

BACKGROUND：Previous studies have demonstrated that anti-inflammatory actions of bone marrow mesenchymal stem cells（BMSCs） could ameliorate severity of acute lung injury.However,only a few studies have addressed BMSCs transplantation for asthma.OBJECTIVE：To study the effect of allogeneic BMSCs transplantation on the serum levels of interleukin-12（IL-12） and IL-4 in chronic asthmatic mice.METHODS：Forty female BALB/c mice were equally randomized into four groups.Mice of normal control and BMSCs control groups received PBS sensitization to arouse mice.On day 21 before suscitation,PBS or BMSCs（30 μL） were injected via the intratracheal injection.Mice of asthma model and BMSCs treatment groups were used to establish chronic asthma using chicken ovalbumin.On day 21 before suscitation,PBS or BMSCs（30 μL） were injected via the intratracheal injection.Serum inflammatory factor levels were measured in mice of each group using enzyme linked immunosorbent assay.RESULTS AND CONCLUSION：There were a great number of inflammatory cell infiltrations around airway and vessel,in conjunction with epithelial proliferation and partial breakage and defluvium,patchy airway occlusion by hyperviscous mucus and hypertrophy and hyperplasia of airway smooth muscle in the asthma model group.However,there was no obvious inflammatory cells infiltration in the airway and airway remodeling both in normal control group and BMSCs control group.Airway inflammation and airway remodeling were significantly relieved in the BMSCs treatment group.Compared to the normal control group and BMSCs control group,low level of IL-12 and high levels of IL-13 and IL-4 were present in the asthma model group.Transplantation of BMSCs significantly elevated level of IL-12 and decreased level of IL-4 in serum.Results suggest that BMSCs for asthma can relieve airway inflammation and airway remodeling by decreasing IL-4 levels and increasing IL-12 levels.