针刺对2型糖尿病大鼠脂肪组织INSR基因表达的影响

目的探讨针刺对2型糖尿病（type 2 diabetes mellitus，T2DM）机体脂肪组织胰岛素受体（insulin receptor，INSR）基因表达的影响。方法给食源性肥胖大鼠腹腔注射小剂量链脲佐菌素复制T2DM模型，随机分为针刺组、优降糖组和模型组，设正常对照组。处理4周后，采用罗氏活力型血糖仪测空腹血糖（fasting blood sugar，FBS），采用放射免疫法检测空腹胰岛素（fasting insulin，FINS），采用原位杂交检测脂肪细胞INSR mRNA表达，计算胰岛素敏感性指数（insulin sensitivity index，ISI）和稳态模型评估胰岛素抵抗（homeostasis model assessment of insulin resistance，Homa—IR）指数。结果与正常组比较，模型组FBS、FINS、ISI、Homa—IR水平显著上升（P〈0．01），INSR基因水平表达显著降低（P〈0．01）；与模型组比较，治疗后优降糖组和针刺组FBS、FINS、ISI、Homa—IR显著下降（P〈0．05，或P〈0．01），INSR基因表达显著升高（P〈0．01）。与优降糖组比较，治疗后针刺组FINS水平显著降低，JNSR基因表达显著升高（P〈0．01）。结论T2DM大鼠存在脂肪细胞，NS尺基因表达低下，针刺和优降糖均可上调T2DM大鼠脂肪细胞INSR基因表达，针刺改善T2DM大鼠脂肪细胞INSR基因表达的作用优于优降糖。

Effect of Acupuncture on Insulin Receptor Gene Expression of Adipose Tissue in Type 2 Diabetes Mellitus

Objective To observe effects of acupuncture on the expression of insulin receptor gene(INSR) mRNA of adipocytes in rats with type 2 diabetes mellitus(T2DM).Methods The rat model of T2DM was induced by intraperitoneal injection of small dosage streptozotocin in rats with diet-induced obesity.Then these T2DM rats were averagely randomized into acupuncture group,Glibenclamide group and model group,and normal rats were used as control.After treatment for 4 weeks,fasting blood sugar(FBS) was assayed with active ...