Measurement of total serum protein by near-infrared reflectance spectroscopy |
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| Authors: | A W van Toorenenbergen B G Blijenberg B Leijnse |
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| Affiliation: | Department of Clinical Chemistry, Erasmus University/University Hospital Rotterdam-Dijkzigt, The Netherlands. |
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| Abstract: | This study describes the measurement of total protein in serum by near-infrared reflectance spectroscopy. With an algorithm, generated by the calibration procedure, the protein content of serum samples was calculated from absorbance data at various wavelengths in the near-infrared. A good correlation (r = 0.993) was found between near-infrared reflectance spectroscopy measurement of serum protein and analysis by the biuret reaction. |
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