| 考虑热量和压力功影响的氢网络扩展矩阵法 |
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| 引用本文： | 刘桂莲,周华,温光林. 考虑热量和压力功影响的氢网络扩展矩阵法[J]. 医学教育探索, 2013, 0(2): 143-146 |
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| 作者姓名： | 刘桂莲 周华 温光林 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237;华东理工大学生物反应器工程国家重点实验室，上海 200237 |
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| 基金项目： | 国家重点基础研究发展（973）计划(2012CB720500)；国家自然科学基金（21076168，20936004） |
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| 摘 要： | 以解藻酸弧菌株（Vibrio alginolyticus）ATCC 17749为研究对象，利用生物信息学寻找并预测得到5个可能的海藻酸裂解酶基因algV1、 algV2、 algV3、 algV4和 algV5。通过构建5个以pET 28a(+)为载体的大肠杆菌表达质粒pET28a algV1、pET28a algV2、pET28a algV3、pET28a algV4和pET28a algV5，实现了5个基因的异源表达，并经海藻酸裂解酶定量和定性的活性分析，确定5个基因的编码产物都具有海藻酸裂解酶活性，其中重组的algV1、 algV2和 algV3为胞外酶，algV4和algV5为胞内酶。
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| 关 键 词： | 解藻酸弧菌ATCC 17749； 海藻酸裂解酶； 大肠杆菌； 异源表达； 酶活测定 |
| 收稿时间： | 2012-07-20 |
Expansion Martix Method for Hydrogen Network Integration Considering Heat and Pressure Work |
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| LIU Gui-lian,ZHOU Hua and WEN Guang-lin. Expansion Martix Method for Hydrogen Network Integration Considering Heat and Pressure Work[J]. Researches in Medical Education, 2013, 0(2): 143-146 |
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| Authors: | LIU Gui-lian ZHOU Hua WEN Guang-lin |
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| Affiliation: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | In the hydrogen network, the hydrogen transfer is accompanied by the heat and work transfer. Therefore, these three factors should be considered together. The impact of heat and work on the hydrogen network integration is relatively smaller than that of hydrogen transfer. Based on this, the expansion matrix method for hydrogen network integration considering heat and pressure work is proposed for the first time. In this method, the match of hydrogen has higher priority than the heat and work transfer. The hydrogen network of a refinery is analyzed by the proposed method, and its optimal network is developed. |
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| Keywords: | Vibrio alginolyticus ATCC 17749 alginate lyases Escherichia coli heterologous expression activity determination |
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