bFGF复合骨基质明胶在兔股前区促血管化作用

 目的探讨成纤维细胞生长因子（bFGF）对骨基质明胶（BMG）包埋血管束在兔股前内侧区的促血管化作用，为解决组织
工程骨的血管化问题奠定实验基础。方法制备兔BMG。将36 只兔随机分为A、B、C 3 组，每组12 只。于股骨前内侧膝关节
上方做一纵行切口。A 组：bFGF+血管束+BMG；B 组：血管束+BMG；C 组：BMG，分别于术后4 周、8 周、12 周给各组动物灌注墨
汁后处死，取出植入物，做透明标本进行大体观察，并做Masson 染色进行组织学观察和定量分析。结果组织学观察表明：术
后12 周，A 组的新生血管排列较有序，B 组的新生血管逐渐趋向于有序排列，C 组新生血管排列杂乱，主要集中于材料边缘，中
央较少。血管面积定量分析：各个时间点上A组血管面积均最大，与B组、C组之间差异有统计学意义（ P<0.05）。新生骨面积
定量分析：各个时间点上A 组新生骨面积均最大，与C 组差异有统计学意义（ P<0.05）。结论bFGF对BMG 包埋血管束有促
血管化的正协同作用。血管化程度越高，新生骨面积越大，新生骨面积比与血管化程度呈正相关。

The Promoting Role of bFGF on the Vascularization of Bone Matrix Gelatin Embedded Vascular Bundles in Rabbit Femoral Anteriomedialis

Objective To investigate the role of basic fibroblast growth factor（bFGF）on the vascularization of bone matrix gelatin（BMG）
embedded vascular bundles in femoral anteriomedialis in the rabbits. Methods A longitudinal incision was done at the hyper-knee joint of
femoral anteriomedialis in the Japan big ears white rabbit. Thirty six rabbits were divided into group A （n =12，arteria saphena and vena
saphena were liberated and embeded to the groove of BMG soaked by 2 μg/ml bFGF0，group B （n =12，arteria saphena and vena saphena
were liberated and embeded to the groove of BMG untreated），and group C （n =12，the BMG untreated were directly implanted）. The rab-bits were sacrificed at 4 weeks，8 weeks and 12 weeks after the surgery respectively by perfusing ink. After the implants were dislodged，
transparent specimens were made and Masson stained for histological observations and quantitative analysis. Results After 12 weeks of op-
eration，the neovascularization arranged in an ordered manner in group A，gradually trended to be orderly in group B，and were cluttered
mainly on the edge of the implants in group C. The osteogenic and neovascularization areas of group A were the largest on each time point.
Conclusion bFGF could promote the vascularization of BMG embedded vascular bundle. There was a positive correlation between osteoge-nesis and vascularization.