吲哚美辛、组胺- 吲哚美辛对鼠Lewis 肺癌凋亡诱导作用的实验研究

 目的探讨吲哚美辛（IN）、组胺-吲哚美辛（his-IN）对Lewis肺癌荷瘤小鼠的肺癌生长的抑制及凋亡的效果。方法处死
Lewis 肺癌荷瘤小鼠后，制成肿瘤细胞悬液，接种于昆明小鼠的左腋下，待肿瘤长至1.0 cm 左右，将其分为5 组，具体为吲哚美
辛（IN 组）3.0 mg/kg 组和3.5 mg/kg 组，组胺-吲哚美辛（his-IN 组）3.0 mg/kg 组和3.5 mg/kg 组及生理盐水对照组，用药治疗10 d
结束后，以抑瘤率、流式细胞仪测定肿瘤细胞凋亡峰及凋亡率、放免法测定肿瘤坏死因子α（TNF-α）评价疗效。结果his-IN 组
与IN组的TNF-α含量、抑瘤率及凋亡率明显高于对照组，而且3.5 mg/kg his-IN 组要明显好于其他各组；通过流式细胞仪发现
不同治疗组都在G0/G1峰前出现“亚二倍体峰”，并且his-IN 组出现要比IN 组早，且凋亡细胞数多，其中3.5 mg/kg his-IN 治疗组
更明显。结论IN、his-IN 可以抑制肿瘤的增长及促进肿瘤细胞的凋亡，其中3.5 mg/kg his-IN 组抑制肿瘤及促进肿瘤细胞凋亡
作用更强。

Experimental Study of the Apoptosis Inducing Effect of Histamin-indomethacin on Lewis Lung Cancer in Mice

Objective To study the apoptosis-inducing effect of histamin-indomethacin（his-IN）on Lewis lung cancer cell in mice. Meth-
ods The suspension of tumor cells from Lewis lung cancer mice was inoculated into the left axilla of Kunming mice. When the tumor grew
to 1 cm in diameter，the mice were divided into 5 groups and received corresponding treatment3.0 mg/kg indomethacin（IN）group，3.5 mg/
kg IN group，3.0 mg/kg his-IN group，3.5 mg/kg his-IN group，and saline control group. Ten days later，the therapeutic effects were evaluated
by the tumor inhibitory rate，the apoptotic peak and rate of tumor cells were measured by flow-cytometry（FCM），and tumor necrosis factor α
（TNF-α）level was estimated by radio-immunity method. Results TNF-αlevel，the tumor inhibitory rate and the apoptosis rate of his-IN
group and IN group were significantly higher than those of the control group，and those of 3.5 mg/kg his-IN group was the highest. In the
groups of his-IN or IN treatment，an apoptotic peak was found before G0/G1 phase，and the peak in his-IN group appeared earlier than IN
group. Conclusion IN and his-IN could resist the growth of the tumor and promote cell apoptosis of Lewis lung cancer in mice.