肿瘤坏死因子α+489G/A基因多态性与慢性阻塞性肺疾病的相关性研究

目的 探讨肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)+489基因多态性与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)易感性的关系.方法 用聚合酶链反应-限制性片段长度多态性技术对55例COPD患者、47例对照组(性别、年龄和吸烟史配对)和55名健康查体组的TNF-α+489G/A基因多态性进行检测,并对其易感性进行分析.结果 COPD组、对照组及健康查体组携带GA/AA基因型的发生率分别为23.6%、14.3%和9.1%,3组间差异无统计学意义(P＞0.05).当COPD组与健康查体组比较,携带等位基因A的人群更易发生COPD(P＜0.05).COPD组携带GA/AA基因型患者肺源性心脏病的发病率明显高于携带GG基因型的患者(P＜0.05).结论 在TNF-α+489位点上携带等位基因A的人群可能是发生COPD的一个高危因素,且预后不佳.

Relationship between gene polymorphisms of tumour necrosis factor-α+489G/A and chronic obstructive pulmonary disease

Objective To study the relationship between tumor necrosis factor-α+489G/A gene polymorphisms and susceptibility to development of chronic obstructive pulmonary disease(COPD).Methods Polymerase chain reaction-restriction fragment length polymorphism method was used to detect the polymorphism of TNF-α+489G/A gene in 5 5 cases of COPD,47 cases of sex,age and smoke history matched controls and 55 cases of body-check healthy subjects and their susceptibility to development of COPD was analyzed.Results The frequencies of GA/AA genotype in COPD group,control group and body-check healthy group were 23.6%,14.3%and 9.1%,respectively.There were not statistic difference between the groups(P＞0.05).Statistical analysis showed that COPD group had a significantly higher allele A frequency than that of body-check healthy group(P＜0.05).The prevalence of chronic pulmonary heart disease in the GA/AA genotype was higher than the GG genotype in COPD group.Conclusions The peoples who carry allele A in TNF-α+489 may increase the risk of COPD and poor progress.