| 几种吡咯里西啶类生物碱对肝细胞毒性的探讨 |
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| 引用本文： | 季莉莉,檀爱民,汤俊,张勉,王峥涛. 几种吡咯里西啶类生物碱对肝细胞毒性的探讨[J]. 中国天然药物, 2004, 2(4): 239-241 |
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| 作者姓名： | 季莉莉 檀爱民 汤俊 张勉 王峥涛 |
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| 作者单位： | 中国药科大学生药研究室,南京,210038 |
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| 基金项目： | 国家杰出青年基金项目 (No .3 982 5 12 9)，国家自然科学基金项目 (No .3 0 2 70 15 7)资助~~ |
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| 摘 要： | 目的：考察几种吡咯里西啶类生物碱体外实验中对肝细胞的毒性。方法：用MTF法检测了6种吡咯里西啶类生物碱clivorine，monocrotaline，isoline，LP-01(yamataimine)，LP-02(12-acetyl yamataimine)，LJ-09(platyphylline)对人正常肝细胞株L-02细胞活性的影响，对于其中有毒性的clivorine又用结晶紫染色的方法进一步确证其毒性。结果：Clivorine对肝细胞具有强烈的毒性作用，未发现其它几种吡咯里西啶生物碱对肝细胞的毒性。结论：Clivorine体外对肝细胞具毒性作用。
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| 关 键 词： | 吡咯里西啶类生物碱 Clivorine 肝细胞毒性 MTT法 结晶紫染色法 实验方法 |
| 文章编号： | 1672-3651(2004)04-0239-03 |
| 修稿时间： | 2003-12-09 |
Toxicity of Several Pyrrolizidine Alkaloids on Hepatocytes |
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| JI Li-Li,TAN Ai-Min,TANG Jun,ZHANG Mian,WANG Zheng-Tao. Toxicity of Several Pyrrolizidine Alkaloids on Hepatocytes[J]. Chinese JOurnal of Natural Medicines, 2004, 2(4): 239-241 |
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| Authors: | JI Li-Li TAN Ai-Min TANG Jun ZHANG Mian WANG Zheng-Tao |
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| Affiliation: | JI Li-Li,TAN Ai-Min,TANG Jun,ZHANG Mian,WANG Zheng-TaoDepartment of Pharmacognosy,China Pharmaceutical University,Nanjing 210038,China |
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| Abstract: | AIM: To test the toxic effects of clivorine,monocrotaline,isoline,platyphylline,yamataimine and 12-acetyl yamataimine pyrrolizidine alkaloids isolated from Ligularia and Monocrotalia on hepatocytes.METHOD: Using MTT experiment to test the effect of these pyrrolizidine alkaloids on hepatocytes.Using crystal violet staining assay to make a further validation of the toxic effect of clivorine.RESULT:Clivorine had toxic effect on hepatocytes and other pyrrolizidine alkaloids had no effect on hepatocytes.The crystal violet staining assay confirmed clivorine's toxic effect.CONCLUSION:Clivorine has direct toxic effect on hepatocytes. |
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| Keywords: | Pyrrolizidine alkaloids clivorine MTT crystal violet staining |
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