mmu-miR-3475-3P在心脏发育中的生物信息学分析

目的 探讨mmu--miR-3475-3P可能参与的生物学过程及信号通路.方法 用实时荧光定量PCR方法测定mmu-miR-3475-3P在小鼠胚胎心脏和成熟心脏中的表达.再综合应用TargetScan、miRDB、miRanda等常用microRNA在线数据库对miR3475-3p进行靶基因预测,对所得靶基因进行基因功能注释(Go)和信号通路分析.结果 mmu-miR-3475-3P在小鼠胚胎心脏和成熟心脏中存在明显的表达差异,运用TargetScan、miRDB、miRanda对miR3475-3P进行生物信息学分析发现该microRNA可能调控441个靶基因.结论 mmu-miR-3475-3P在小鼠胚胎心脏高表达,其预测的靶基因富集于多个信号通路及细胞生物学过程.

Bioinformatics analysis for mmu-miR-3475-3P in cardiac development

Objective To investigate the mmu-miR-3475-3P possible participating in biology process and signal pathway.Methods The expression of mmu-miR-3475-3P in the mature mouse heart and in the embryonic mouse heart was measured by qRT-PCR.The target genes were predicted through comprehensively using the common microRNA on-line databases such as TargetScan,miRDB and miRanda,and then the obtained targeted genes were performed the gene function annotation and signal pathway analysis.Results There was significant difference between the expression of mmu-miR-3475-3P in the embryonic mouse heart and the expression in the mature heart.Bioinformatics analysis by using TargetScan,miRDB and MiRanda on miR3475-3P revealed that microRNA was likely to regulate 441 target genes.Conclusion mmu-miR-3475-3P is highly expressed in the embryonic mouse heart.The target genes predicted by mmu-miR-3475-3P are enriched in multiple signal pathways and cellular biological processes.