重庆地区婴幼儿和儿童血凝五项参考区间的调查

目的 建立重庆地区婴幼儿和儿童血凝检查项目的参考区间,以更好地服务于临床.方法 采用Sysmex CS5100、Sysmex CSA2000全自动血凝分析仪检测重庆地区经健康筛查的5 119例儿童的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白原(FIB)、凝血酶时间(TT)、血浆D-二聚体(D-D)血凝五项.将所有儿童根据年龄分为6组:新生儿(0～＜15 d)、新生儿(15 d至小于1个月)、婴儿(1～＜7个月)、幼儿(7个月至小于3岁)、儿童(3～＜7岁)、儿童(7～15岁)组,进行比较分析.正态分布的资料按照(x)±1.96s确定参考区间,偏态分布的资料按照P2.5～P975确定参考区间.结果 本室血凝五项参考区间,PT:11.1～20.2 s(0～＜15 d)、10.7～15.8 s(15 d至小于1个月)、9.9～13.5 s(1个月至15岁);APTT:28.8～91.6 s(0～＜15 d)、33.3～64.2 s(15d至小于1个月)、24.0～53.2 s(1～＜7个月)、21.2～39.2 s(7个月至15岁);FIB:1.45～3.52 g/L(0～＜3岁)、1.50～3.52 g/L(3～15岁);TT:15.4～26.3 s(0～＜15 d)、14.9～21.4 s(15 d至小于7个月)、14.7～20.1 s(7个月至小于3岁)、14.9～19.3 s(3～15岁);D-D:0.1～5.1 mg/L(0～＜15 d)、0.2～2.2 mg/L(15 d至小于1个月)、0.1～1.1 mg/L(1～＜7个月)、0.1～1.4 mg/L(7个月至小于3岁)、0.1～1.3 mg/L(3～＜7岁)、0～1.0 mg/L(7～15岁).结论 初步建立了重庆地区婴幼儿和儿童血凝五项(PT、APTT、FIB、TT、D-D)的参考区间,为该地区婴幼儿及儿童相关疾病的诊疗提供了更准确的依据.

Investigate on reference intervals of five indexes of blood coagulation tests among infants and children in Chongqing

Objective To establish children's independent normal reference intervals of five coagulation indexes in Chongqing,in order to better provide references for clinic.Methods The plasma samples of 5 119 healthy children were collected and used to detecting prothrombin time (PT),activated partial thromboplastin time (APTT) and fibrinogen (FIB),thrombin time (TT) and D-Dimer (D-D) via Sysmex CS5100 and Sysmex CSA2000.According to ages,the children were divided into 6 groups:newborn (at 0 to less than 15 days of age),newborn (at 15 days to less than 1 month of age),infant (at 1 to less than 7 months of age),children (at 7 months to less than 3 years of age),children (at 3 to less than 7 years of age),children (at 7 to 15 years of age),and comparatively analysed.For normal distribution data,(x)± 1.96s was used to calculate the reference intervals;while for skewness distribution data,the reference intervals was calculated by 95 ％ reference interval according to the percentile of P2.5 and P97.5.Results The normal reference intervals of five coagulation indexes in this clinical laboratory were as follows:PT 11.1-20.2 s (at 0 to less than 15 days of age),10.7-15.8 s (at 15 days to less than 1 month of age),9.9-13.5 s (at 1 month to 15 years of age);APTT 28.8-91.6 s (at 0 to less than 15 days of age),33.3-64.2 s (at 15 days to less tha 1 month of age),24.0-53.2 s (at 1 to less than 7 months of age),21.2-39.2 s (at 7 months to less tha 15 years of age);FIB 1.45-3.52 g/L (at 0 to less than 3 years of age),1.50-3.52 g/L (at 3 to 15 years of age);TT 15.4-26.3 s (at 0 to less than 15 days of age),14.9-21.4 s (at 15 days to less than 7 months of age),14.7-20.1 s (at 7 months to less than 3 years of age),14.9-19.3 s (at 3 to 15 years of age);D-D 0.1-5.1 mg/L (at 0 to less than 15 days of age),0.2-2.2 mg/L (at 15 days to less than 1 month of age),0.1-1.1 mg/L (at 1 to less tha 7 months of age),0.1-1.4 mg/L (at 7 months to less than 3 years of age),0.1-1.3 mg/L (at 3 to less than 7 years of age),0-1.0 mg/L (at 7 to 15 years of age).Conclusion The normal reference intervals of five coagulation indexes,including PT,APTT,FIB,TT and D-D,are established,which provide more accurate references for the diagnosis of related diseases in infants and children in this area.