抑癌基因PTEN在肝细胞癌中的表达及其意义

目的：探讨肝细胞癌（HCC）组织中抑癌基因PTEN蛋白的表达及其意义。方法：应用免疫组织化学SP法，检测53例HCC组织和癌周组织中PTEN蛋白的表达水平。结果：53例HCC中PTEN蛋白阳性率（52．8％）和阳性强度明显低于癌旁组织（92．5％）（P〈0．01，P〈0．05）；HCC组织中PTEN蛋白的表达与HCC的分化程度密切相关（P〈0．05），HCC分化愈差，PTEN蛋白表达愈弱；PTEN蛋白表达还与癌拴形成有关（P〈0．005）。结论：PTEN的异常表达在HCC发生、发展过程中可能起重要作用，其表达水平有可能作为反映HCC进展和预后的生物学指标。

Expression of tumor suppressor gene PTEN in human hepatocellular carcinoma(HCC) and its clinical significance

Objective:To investigate the expression of phosphatase and tensin homologue deleted on chromosome 10(PTEN) in human hepatocellular carcinoma(HCC) and its clinical significance.Methods:The expression of PTEN was detected by immunohistochemistry SP method in 53 cases of hepatocellular carcinoma and their pericarcinomatous tissue.Results:The positive rate (52.8%) and expression intensity of PTEN in human HCC were lower and weaker than these in the pericarcinomatous liver tissue(92.5%)(P<0.01,P<0.05),and the expression level of PTEN was significantly related to the differentiation of HCC,the weaker the expression of PTEN protein.The positive rate was relative to the presence of tumor thrombus(P<0.005).Conclusion:The abnormal expression of PTEN may play an important role in carcinogenesis and development of HCC,the expression level of PTEN may be a molecular-biological indicator in forecasting the progress and progrosis of HCC.