维生素D受体基因FokⅠ及BsmⅠ多态性与老年男性2型糖尿病脂代谢异常的相关性 |
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引用本文: | 夏征,胡亚卓,张红红,韩志涛,白洁,傅淑宏,邓新立,何耀. 维生素D受体基因FokⅠ及BsmⅠ多态性与老年男性2型糖尿病脂代谢异常的相关性[J]. 南方医科大学学报, 2014, 34(11): 1562-1568 |
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作者姓名: | 夏征 胡亚卓 张红红 韩志涛 白洁 傅淑宏 邓新立 何耀 |
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作者单位: | 解放军总医院//解放军医学院老年医学研究所//衰老及相关疾病研究北京市重点实验室;解放军总医院//解放军医学院南楼检验科 |
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摘 要: | 目的探讨维生素D受体(VDR)基因FokⅠ和BsmⅠ位点多态性与北京地区汉族老年男性2型糖尿病脂代谢异常的相关 性。方法采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)和基因测序技术,检测328例北京汉族老年男性VDR 基因FokⅠ和BsmⅠ位点基因型和等位基因频率,同时与相关临床指标进行比较。其中2型糖尿病患者(DM组)237例与正常对 照(NC组)91例。根据血脂情况将DM组分为非血脂异常组(DO组)134例和血脂异常组(DH组)103例。结果VDR基因Fok Ⅰ和BsmⅠ位点基因频率分布符合Hardy-Weinberg平衡,具有群体代表性。VDR基因FokⅠ位点F等位基因频率在DM组较 NC组显著升高(χ2=3.873,P=0.049,OR=1.439,95% CI:1.001-2.071)。在显性模型下,DM组FF基因型频率较NC组显著升高(χ2= 5.057,P=0.025,OR=1.756,95% CI:1.072-2.875);DH组FF基因型频率较NC组显著升高(χ2=6.168,P=0.013,OR=2.06,95% CI: 1.161-3.663);其余各组组间比较,基因型及等位基因频率差异均无统计学意义(P>0.05);与Ff+ff基因型比较,FF基因型人群的 平均舒张压水平显著降低,而餐后2 h血糖、甘油三酯、尿酸的水平显著升高,差异有统计学意义(分别是P=0.039;P=0.035;P= 0.049;P=0.031)。BsmⅠ位点基因型及等位基因频率在各组间的差异均无统计学意义(P>0.05)。bb基因型人群血肌酐水平显 著高于BB+Bb基因型,差异有统计学意义(P=0.011)。结论VDR基因FokⅠ位点多态性可能是老年男性2型糖尿病合并脂代 谢异常的危险因素,而BsmⅠ多态性可能与老年男性2型糖尿病无相关。
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关 键 词: | 维生素D受体 2型糖尿病 血脂异常 单核苷酸多态性 |
Association of vitamin D receptor Fok I and Bsm I polymorphisms with dyslipidemias inelderly male patients with type 2 diabetes |
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Abstract: | Objective To assess the association of vitamin D receptor (VDR) gene Fok I and Bsm I polymorphisms with dyslipidemia in elderly male patients with type 2 diabetes of Han nationality. Methods A total of 328 elderly male residents of Han nationality in Beijing, including 237 type 2 diabetic patients and 91 healthy control subjects, were enrolled in this study. The diabetic patients were divided into non-dyslipidemia group (DO group, n=134) and dyslipidemia group (DH group, n= 103). All the participants were genotyped for Fok I and Bsm I polymorphisms in VDR gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technology, and the results were compared with their clinical characteristics. Results For Fok I, the frequency of F allele was significantly higher in the diabetic patients than in the control group (χ2=3.873, P=0.049, OR=1.439, 95% CI: 1.001-2.071). In the dominant model, the frequency of FF genotype was significantly higher in the diabetic group (χ2=5.057, P=0.025, OR=1.756, 95% CI: 1.072-2.875) as well as in DH group (χ2=6.168, P=0.013, OR=2.06, 95% CI: 1.161-3.663) than in the control group. There was no significant differences in the genotype frequency or allele distribution in other paired groups (P>0.05). Compared with Ff + ff genotype, FF genotype was associated with a significantly decreased average diastolic blood pressure (P=0.039) but significantly increased postprandial blood glucose (P=0.035), triglycerides (P=0.049) and uric acid (P=0.031). No significant difference was detected in genotype frequency or allele distribution of Bsm I polymorphisms between the groups (P>0.05); serum creatinine levels were significantly higher in bb genotype than in BB + Bb genotype group (P=0.011). Conclusion VDR gene Fok I polymorphisms may be a risk factor for dyslipidemia in elderly male patients with type 2 diabetes among Chinese Han population, where Bsm I polymorphisms are not associated with diabetic dyslipdiemia.
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