A high resolution CEPH crossover mapping panel and integrated map of chromosome 11

High resolution (0.1 cM) CEPH crossover mapping panels were constructed forchromosome 11. These panels will facilitate a transition from top- downphysical and genetic mapping strategies to integrated breakpoint mappingstrategies. Novel methods, which differ from other methods in overcomingthe limitations of incomplete heterozygosity and variable marker density,were developed for creating the panels and integrated maps. This made itpossible to identify and sublocalize the majority of crossovers in 61families. The panels were used to map 139 microsatellite markers. Asemi-integrated map and a fully-integrated map were constructed bycombining these data with data from CEPH 7.1 and then integrating data fromthe radiation hybrid (RH) map. Genetic lengths estimated from the mappingpanels were similar to the estimates obtained when all recombinant andnon-recombinant offspring were included (189.4 cM in females and 126.1 cMin males), indicating that genetic distances are stable at this high markerdensity. The maps have a cM density of 0.62. The distance between orderedmarkers is 1.39-2.92 cM depending on the criterion for order and the extentof map integration. The 2D maps provide the resolution and flexibilityneeded to enhance current applications such as positional cloning andmapping complex disorders; while the mapping panels will greatly improvethe resolution, reliability and efficiency of future genetic mapping.