原因不明复发性流产患者蜕膜组织中Th1/Th2比率与B7-1、B7-2的相关性

目的:探讨原因不明复发性流产患者蜕膜组织中Th1/Th2比率与巨噬细胞B7-1、B7-2表达水平的相关性,分析蜕膜组织中共刺激分子B7-1、B7-2及Th1/Th2比率失衡在原因不明复发性流产发病中的作用。方法:用双荧光标记流式细胞技术检测原因不明复发性流产妇女(流产组)和正常妊娠妇女(对照组)的蜕膜组织中Th1/Th2比率和巨噬细胞B7-1、B7-2的表达水平。结果:流产组妇女蜕膜组织中Th1/Th2比率(20.41±3.32)、巨噬细胞B7-1的表达水平(21.57±1.70)%明显高于对照组(P<0.01);流产组妇女蜕膜组织中巨噬细胞B7-2的表达水平(27.82±2.28)%明显低于对照组(P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-1的表达水平之间存在显著正相关(r=0.8663,P<0.01);流产组妇女蜕膜组织中Th1/Th2比率与蜕膜组织中巨噬细胞B7-2的表达水平之间存在显著负相关(r=-0.9016,P<0.01)。结论:原因不明复发性流产患者蜕膜组织中B7-1表达水平增高、B7-2表达水平降低与蜕膜组织中Th1/Th2比率异常有密切的关系,可能是导致原因不明复发性流产发生的重要因素。

Study on the relationship between Th1/Th2 and B7-1,B7-2 in uterine decidua in unexplained recurrent spontaneous abortion

Objective:To evaluate the relationship between Th1/Th2 and B7-1,B7-2 in uterine decidua in unexplained recurrent spontaneous abortion(URSA)and to investigate the function of them in the pathogenesis.Methods:The expression of Th1/Th2 and B7-1,B7-2 in uterine decidua from 20 patients with URSA,19 normal pregnancy women were determined by double-label flow cytometric technique.Results:The expression of Th1/Th2(20.41±3.32)and B7-1(21.57±1.70)% was higher significantly in patients with URSA than that in normal pregnancy women(P<0.01).The expression of B7-2(27.82±2.28)% was lower significantly in patients with URSA than that in normal pregnancy women(P<0.01).There were positive correlated between Th1/Th2 and B7-1(r=0.866 3,P<0.01).There were negative correlated between Th1/Th2 and B7-2(r=-0.901 6,P<0.01).Conclusion:Overexpression of B7-1 and inhibition of B7-2 lead to the imbalance of Th1/Th2,and it might be the reason of URSA.