超高效液相色谱法测定参杞通脉胶囊中人参皂苷Rg1、Re和Rb1含量

目的建立测定参杞通脉胶囊中人参皂苷Rg1、Re和Rb1含量的超高效液相色谱(UPLC)法。方法色谱柱为Acquity UPLC HSS T3色谱柱(50 mm×2.1 mm,1.8μm),流动相为乙腈-水(梯度洗脱),流速为0.4 mL/min,柱温为28℃,检测波长为203 nm,进样量为5μL。结果人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1进样量分别在0.0754~0.6032μg,0.0523~0.4184μg,0.0760~0.6084μg范围内与峰面积线性关系良好,平均回收率分别为97.90%,97.41%,98.02%,RSD分别为1.06%,1.73%,0.91%(n=6)。结论该方法操作简单,分离度高,回收率高,可用于参杞通脉胶囊的质量控制。

Determination of Ginsenoside Rg1,Re and Rb1 in Shenqi Tongmai Capsules by UPLC

Objective To establish an ultra performance liquid chromatography(UPLC)method for the determination of ginsenoside Rg1,Re and Rb1 in Shenqi Tongmai Capsules.Methods The chromatographic column was ACQUITY UPLC HSS T3(50 mm×2.1 mm,1.8μm);the mobile phase was acetonitrile-water,and gradient elution was performed;the flow rate was 0.4 mL/min,the column tem-perature was 28℃,the detection wavelength was 203 nm,and the injection volume was 5μL.Results The linear relationship of gin-senoside Rg1 in the range of 0.0754-0.6032μg,ginsenoside Re in the range of 0.0523-0.4184μg,ginsenoside Rb1 in the range of 0.0760-0.6084μg was good.The average recoveries were 97.90%,97.41%,98.02%,RSDs were 1.06%,1.73%and 0.91%,respectively(n=6).Conclusion The method is simple in operation,high in separation and good in recovery.It is suitable for the quality control of Shenqi Tongmai Capsules.