黄药子配伍甘草对胃癌细胞增殖及侵袭的影响

目的探讨黄药子配伍甘草对人胃癌细胞SGC7901增殖及侵袭的影响。方法体外培养胃癌细胞SGC7901,以黄药子配伍甘草含药血清处理,采用CCK-8法检测细胞增殖,Transwell试验检测细胞侵袭性,实时荧光定量(qRT-PCR)检测上皮间质转化(EMT)相关基因的表达。结果中药含药血清能抑制胃癌细胞SGC 7901增殖,且抑制作用随着药物浓度的增加而加强(P<0.05);中药组Transwell小室穿膜细胞数随着浓度的增加而减少(P<0.05);qRT-PCR结果显示,空白组E钙黏蛋白表达较其他组低,不同浓度中药组E钙黏蛋白表达随着浓度的增加而增加;中药组较空白组N钙黏蛋白和金属基质蛋白酶(MMP)-9的表达均降低,随着中药浓度的增加而显著下降(P<0.05)。结论黄药子配伍甘草能抑制胃癌细胞SGC 7901的增殖,并通过抑制EMT而降低癌细胞的侵袭性。

Effects of Dioscorea Bulbifera and Glycyrrhiza Uralensis on Proliferation and Invasion in Gastric Cancer Cell

Objective To investigate the synergistic effects of Dioscorea bulbifera and Glycyrrhiza uralensis on proliferation and invasion in gastric cancer cell line SGC7901.Methods The gastric cancer cells SGC7901 were used to treat with the drug-containing serum with the combination of Dioscorea bulbifera and Glycyrrhiza uralensis.The cell proliferation and invasion were detected by using the CCK-8 method and transwell.The expression of epithelial mesenchymal transition(EMT)-related genes were detected by real-time quantitative polymerase chain reaction(qRT-PCR).Results The serum containing traditional Chinese medicine(TCM)could inhibit the proliferation of gastric cancer cell line SGC 7901,and with the increase of drug concentration,the inhibition effect was stronger(P<0.05).The numbers of transwell cells in TCM group were decreased with the increase of drug concentration(P<0.05).The results of PCR showed that the expression of E-cad in control group was lower than that in other groups.The expression of E-cad in different concentration groups increased with the increase of drug concentration.The expression of N-cad and MMP-9 in TCM group was lower than those in control group and decreased significantly with the increase of drug concentration(P<0.05).Conclusion Dioscorea bulbifera and Glycyrrhiza uralensis can inhibit the proliferation of gastric cancer cell line SGC 7901 and decrease the invasiveness of gastric cancer by inhibiting EMT.