三七皂苷Rg1对t-PA和PAI-1水平的影响

目的探讨三七皂苷Rg1对组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物(PAI-1)水平的影响及其作用机制。方法运用发色底物法测定三七皂苷-Rg1在家兔体内、外对血浆纤溶酶原激活物(t-PA)和血浆或血小板释放的纤溶酶原激活物抑制物(PAI-1)水平的影响。结果三七皂苷-Rg1在体外能抑制血浆PAI-1活性,提高血浆t-PA活性,其作用强度呈现出剂量依赖性;静脉内给药显示:三七皂苷-Rg1 30、60、120和240 mg.kg-1组能降低血浆或血小板释放的纤溶酶原激活物抑制物(PAI-1)水平,增强血浆t-PA活性,而且本品还能降低激活的血小板所释放的PAI-1水平。结论三七皂苷Rg1能够有效对抗由于PAI-1活性增高和t-PA活性降低所引起的血栓,这可能是其具有抗血栓作用的有效机制之一。

Effects of ginsenoside-Rg1 on levels of t-PA and PAI-1

Aim To explore the effects and its mechanisms of ginsenoside-Rg1 on level of t-PA and PAI-1.Methods Type 1 plasminogen activator inhibitor(PAI-1) and tissue type plasminogen activator(t-PA) activity in plasma were assayed using chromogenic substrate.Results The results showed that ginsenoside-Rg1 in vitro or in vivo significantly inhibited PAI-1activity,while increased t-PA activity.These effects were concentration-dependent.Intravenous Panax notoginsenoside Rg1 at 30,60,120 and 240 mg·kg~(-1) markedly suppressed PAI-1 level in plasma as well as platelet-released substances stimulated by thrombin,while increased plasma t-PA activity.And release level of PAI-1 owing to blood platelet was greatly decreased by ginsenoside-Rg1.Conclusion Ginsenoside-Rg1 showed potent antithrombosis due to the inhibition of PAI-1 and increase of t-PA.It might also be a advantagous mechanism to its antithrombsis.