持续性牵张力在高糖状态下对子宫平滑肌细胞收缩蛋白表达的影响

目的：探讨持续性牵张力在高糖环境下对大鼠子宫平滑肌细胞催产素受体和前列腺素受体表达的影响。方法：体外培养大鼠子宫平滑肌细胞，高糖作用不同时间后，观察高糖状态下大鼠子宫平滑肌细胞催产素受体和前列腺素受体表达变化情况。对高糖状态下的肌细胞施加持续性牵张力，明确牵张力对肌细胞催产素受体和前列腺素受体表达的影响以及高糖和牵张力之间的协同作用，同时采用晚期糖基化终末产物（ＡＧＥｓ）抑制剂（ｓＲＡＧＥ）拮抗高糖作用作为对照，比较加入抑制剂后，催产素受体和前列腺素受体表达变化情况。结果：单独高糖培养或单独持续性牵张力都可导致大鼠子宫平滑肌细胞裂解液中催产素受体和前列腺素受体表达量上升，二者同时施加，可使催产素受体和前列腺素受体表达进一步上升，并有一定的协同效应。加入sRAGE后均可使催产素受体和前列腺素受体表达部分下降，但仍高于生理糖浓度组。结论：高糖状态及牵张力均可促进肌细胞催产素受体和前列腺素受体表达增加，并有一定协同作用，AGEs参与了这一过程，但并不是唯一途径。

Effect of Continuous Stretch on Contractile Protein Expression of Uterine Smooth Muscle under High Glucose Circumstance

Objective:To analyze the effect of continuous stretch on oxytocin receptors and prostaglandin receptors expression of uterine smooth muscle cells under high glucose circumstance. Methods:Rat uterine smooth muscle cells were separated and cultured under high glucose circumstance for different durations, then we detected oxytocin receptors and prostaglandin receptors expression changes of smooth muscle cells. Continuous stretch was forced on cells under high glucose circumstance to determine synergistic effect of stretch and high glucose. At the same time, as a control team, advanced glycation end products (AGEs) inhibitor (sRAGE) was added to antagonize the effect of high glucose, then evaluate the expression of oxytocin receptors and prostaglandin receptors. Results:Both high glucose circumstance and continuous stretch could lead to oxytocin receptors and prostaglandin receptors expression of rat uterine smooth muscle cells increasing. Exerting high glucose and continuous stretch simultaneously could promote oxytocin receptors and prostaglandin receptors expressions even further and synergistic effect could be detected. After adding sRAGE, oxytocin receptors and prostaglandin receptors expressions decreased to some extend, but not completely, still higher than the normal glucose teams. Conclusions:Both continuous stretch and high glucose could promote oxytocin receptors and prostaglandin receptors expressions and there exist a synergistic effect between them. AGEs was involved in this process, but this is not the only signal pathway.