| 高效液相色谱法测定木樨科苦丁茶中槲皮素的含量 |
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| 引用本文： | 陈艺文,李先荣,倪艳,卢方晋. 高效液相色谱法测定木樨科苦丁茶中槲皮素的含量[J]. 中国中医药信息杂志, 2009, 16(4): 47-48 |
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| 作者姓名： | 陈艺文 李先荣 倪艳 卢方晋 |
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| 作者单位： | 山西省中医药研究院,山西,太原,030012 |
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| 摘 要： | 目的建立测定木樨科苦丁茶中槲皮素的含量测定方法。方法采用高效液相色谱法，色谱柱为Hypersi 1ODS2（4．6mm×250mm，5μm），以甲醇-0．4％磷酸（47：53）为流动相，流速为1mL／min，检测波长为360nm。结果槲皮素的线性范围为0．0504～1．0080gg，r=0．99999；平均回收率为101．61％，RSD=1．75％（n=5）。结论该方法简便、准确，可以作为木樨科苦丁茶的质量控制依据。
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| 关 键 词： | 木樨科苦丁茶 槲皮素 高效液相色谱法 含量测定 |
Determination of Quercetinum in Kuding Cha of Ligustrum by HPLC |
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| Affiliation: | CHEN Yi-wen, LI Xian-rong, NI Yan, et al (Shanxi Academy of TCM, Taiyuan 030012, China) |
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| Abstract: | Objeetlve To set up the determining method of quercetinum in Kuding Cha of Ligustrum by HPLC. Method Hypersil ODS2 chromatographic column （4.6 mm×250 mm, 5 μm） was adopted. Methanol- 0.4% phosphoric acid （47 ： 53） was used as mobile phase, the flow rate was 1 mL/rnin, and the detecting wavelength was 360 nm. Result The linear range of quercetinum was 0.050 4- 1.008 0 lag, r-0.999 99, and the average recovery was 101.61% （RSD= 1.75%, n =5）. Conclusion The method is convenient and accurate, and can used be as basis of quality control for Kuding Cha of Ligustrum. |
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| Keywords: | Kuding Cha of Ligustrum quercetinum HPLC content determination |
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