牛黄及胆红素对三氯乙烯染毒小鼠脂质过氧化的拮抗作用

目的研究牛黄、胆红素对三氯乙烯(TCE)染毒ICR小鼠所致的脂质过氧化的拮抗作用。方法用TCE灌胃染毒ICR小鼠制造脂质过氧化模型,然后分别以牛黄、胆红素灌胃,测定ICR小鼠肝、肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH Px)、过氧化氢酶(CAT)。结果与阴性对照组比,TCE处理组肝、肾组织GSH Px、SOD、CAT活力降低,MDA含量增加,差异均有显著性(P<0.05);牛黄、胆红素染毒与TCE处理组比较,肝、肾组织GSH Px、SOD、CAT活力显著增强(P<0.05),脂质过氧化产物MDA含量减少。结论牛黄和胆红素均能较好地拮抗TCE所引起的ICR小鼠脂质过氧化。

The antagonism of cow-bezoar and bilirubin on the lipid peroxidation of mice induced by trichloroethylene

Objective To study the antagonism of cow-bezoar and bilirubin on the lipid peroxidation of ICR mice induced by trichloroethylene (TCE). Methods The model of lipid peroxidation was established by administering TCE to ICR mice by gavage. The activities of SOD, GSH-Px, CAT, MDA in liver and kidney were then measured. Results The activities of SOD,GSH-Px,CAT were lower and the quantity of MDA was higher in TCE groups, as compared with those in the negative controls, and the differences reached statistically significant level(P<0.05). The activities of SOD,GSH-Px,CAT were higher, while the the quantity of MDA was lower in cow-bezoar and bilirubin groups, as compared with those in the negative controls, and significant differences were also shown (P<0.05). Conclusion Cow-bezoar and bilirubin could inhibit lipid peroxidation on ICR mice induced by TCE.