活性维生素D_3 顺铂对卵巢癌HO-8910细胞株的凋亡研究

目的观察1,25(OH)2维生素D3单独及其与顺铂合用对人卵巢癌细胞株凋亡的影响及Bax蛋白的表达情况。方法四甲基偶氮唑蓝(MTT)法检测细胞抑制率,流式细胞术测定细胞周期及凋亡率,免疫组织化学法检测Bax蛋白表达情况。结果1,25(OH)2维生素3、顺铂均可抑制HO-8910细胞的生长(P<0.01)、均可阻滞细胞于G0/G1期并诱导HO-8910细胞凋亡(P<0.01),当两者合用时上述作用加强,凋亡率明显上升(P<0.01),两者均可上调Bax蛋白表达(P<0.01),两者合用时Bax蛋白表达增强(P<0.01)。结论1,25(OH)2维生素D3与顺铂合用能协同抑制HO-8910细胞的生长并进一步诱导其凋亡。

The effect of 1,25(OH)_2 VitD_3 and DDP on apoptosis of ovarian cancer cell line HO-8910

Objective To study the effect of 1,25(OH)2VitD3 and DDP on growth of HO-8910 human ovarian cells.Methods Cell proliferation was detected by MTT method,distribution of cell cycle and rate of apoptosis were determined by flow cytometry,and the expression of Bax protein was detected by immunohistochemistry.Results The proliferation of HO-8910 cells was inhibited,the cell cycle was arrested in G0/G1 phase and rate of apoptosis was inducted by either 1,25(OH)2VitD3 or DDP alone(P<0.01).Combined use of 1,25(OH)2VitD3 and DDP could increase the inhibitory effect and apoptosis rate(P<0.01).1,25(OH)2VitD3 or DDP alone could up-regulate the Bax protein expression (P<0.01).Combined use of 1,25(OH)2VitD3 and DDP could Bax protein expression(P<0.01).Conclusion 1,25(OH)2VitD3 and DDP synergistically inhibit proliferation and induce apoptosis in ovarian cancer cells.