腺样囊性癌肺转移相关蛋白质的筛选鉴定

目的　初步筛选和鉴定人涎腺腺样囊性癌肺转移相关蛋白质。方法　利用腺样囊性癌细胞株 (ACC 2 )及其肺高转移细胞株 (ACC M) ,通过蛋白质组学方法 ,差减处理 ,发现两细胞株间蛋白质表达的差异 ,并对差异蛋白质点进行分析鉴定。结果　发现ACC 2和ACC M细胞系之间有 12个蛋白质点表达水平明显不同。其中转酮醇酶、v Ha ras蛋白等在ACC 2中低表达 ,在ACC M中高表达 ;肿瘤坏死因子超家族成员 4 (配合基 )在ACC 2中高表达 ,在ACC M中低表达 ,Pirin仅在ACC 2中表达。结论　 12个差异蛋白质可能通过不同途径参与腺样囊性癌肺高转移 ,为研究腺样囊性癌肺转移提供新的线索

Analysis of differentially expressed lung metastasis-associated proteins in Adenoid cystic carcinoma cell lines

Objective To analyze differentially expressed metastasis associated proteins in Adenoid cystic carcinoma cell lines of human salivary gland by proteomics. Methods Protein expression alterations of ACC 2 and ACC M cells were described by 2 D gels. After image analysis by software, proteins of interest were excised from the gels and identified by matrix assisted laser desorption ionization time of flight mass spectrometer. Results 12 protein spots showed significantly differential expression patterns between two cell lines. In the identified protein candidates, transketolase, modulator recognition factor 2, Dim1p homolog, splicing factor ( arginine/serine rich 9) and v Ha ras l oncogene were all lowly expressed in the poorly metastatic ACC 2 cell and significantly upregulated in highly metastatic ACC M cell, while type I collagen pro alpha and tumor necrosis factor (ligand) superfamily member 4 showed a high expression in ACC 2 cells and a low expression in ACC M cells. Pirin (spot 6) just appears in ACC 2 cell and was not detectable in ACC M cell, while retinal homeobox protein was just detected in ACC M cell and did not appear in ACC 2 cell.Conclusions The proteins may be involved in the adenoid cystic carcinoma lung metastasis through different mechanisms. Our work may contribute to discover diagnostic markers and therapeutic targets.