Absence of subviral particles and assembly activity in HeLa cells infected with defective-interfering (DI) particles of poliovirus |
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| Authors: | B A Phillips R E Lundquist J V Maizel |
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| Institution: | 1. Department of Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA;2. Section on Molecular Structure, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20205, USA |
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| Abstract: | HeLa cells infected with defective-interfering (DI) particles of poliovirus were examined for their capacity to synthesize viral proteins, viral-related particles, and the viral factor(s) that promotes the assembly of 14 S particles into empty capsidsin vitro. As reported by C. N. Cole and D. Baltimore (1973,J. Mol. Biol.76, 325–343) cells infected with purified DI particles failed to synthesize the capsid precursor NCVP 1a or any of the capsid polypeptides VP 0, VP 1, VP 2, or VP 3. Consequently, no 14 S particles, empty capsids, or virions were formed at low to moderate multiplicities. Cytoplasmic extracts prepared from cells infected with purified DI particles do not promote the assembly of viral 14 S particles into empty capsids. We conclude that the presence of assembly activity is dependent on the formation of the capsid precursor protein (NCVP-1a) or its cleavage products. |
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| Keywords: | To whom reprint requests should be addressed |
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