Calcium ion uptake induced by cholinergic and α-adrenergic stimulation in isolated cells of rat salivary glands |
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Authors: | H. R. Koelz S. Kondo A. L. Blum I. Schulz |
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Affiliation: | (1) Max-Planck-Institut für Biophysik, Kennedyallee 70, D-6000 Frankfurt am Main, Federal Republic of Germany;(2) Medizinische Klinik, Stadtspital Triemli, Birmensdorfer-Strasse 497, CH-8063, Zürich, Switzerland;(3) School of Medicine, Dept. of Dermatology, Yamagata University, Yamagata City, Japan |
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Abstract: | Summary Adrenaline (10–5 M) and carbamylcholine (10–4 M) stimulate45Ca2+ uptake into isolated cells of rat submandibular gland and parotid glands. In the presence of the -adrenoreceptor blocking agent phentolamine, adrenaline stimulation of45Ca2+ uptake is abolished. The -adrenergic stimulant isoproterenol has no effect on45Ca2+ uptake. Carbamylcholine induced45Ca2+ uptake is inhibited by atropine. The Ca2+ ionophore A23187 stimulates45Ca2+ uptake, whereas dibutyryl cyclic adenosine 3,5-monophosphate and dibutyryl cyclic guanosine 3,5-monophosphate have no effect on45Ca2+ uptake.A graphical analysis of the45Ca2+ uptake curves reveals at least two phases: a fast phase and a slow phase, both of which are stimulated by adrenaline and carbamylcholine.The45Ca-exchangeable pool size is increased by adrenaline and carbamylcholine in both the fast and the slow phases.These results suggest that -adrenergic and cholinergic agonists act by increasing the rate of Ca2+ transfer into the cells of the parotid and submandibular salivary glands most probably through an increase of the cell membrane permeability for Ca2+.Supported by Swiss National Science Foundation Grant No. 3.298.074 |
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Keywords: | Calcium Isolated cells Parotid Submandibular gland Neurotransmitter |
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