乙肝患者血清中HBV C基因启动子变异与病毒含量及前S1抗原的关系

目的 研究HBV C基因启动子(Basic Core Promoter，BCP)基因变异与前S1(Pre-S1)抗原的关系以及BCP基因变异与HBV DNA含量的关系。方法用PCR-微板核酸杂交ELISA技术检测94例HBeAg阳性乙肝患者的BCP中nt1762A-T和nt1764G-A的基因突变，酶联免疫吸附分析(ELISA)检测Pre-S1抗原，PCR荧光定量技术检测HBV DNA。结果在94例HBeAg阳性乙肝患者的血清中，Pre-S1抗原阳性有65例，其中BCP阳性51例，BCP变异率为78．5％；Pre-S1抗原阴性有29例，其中BCP阳性23例，BCP变异率为79．3％。BCP基因突变血清中HBV DNA拷贝数明显高于非BCP基因突变血清中HBV含量。结论 在e抗原阳性乙肝患者中，Pre-S1抗原的存在与BCP的变异无相关性，但BCP基因变异与HBV DNA拷贝数明显相关，可反映乙肝病情的严重程度及其愈后、转归。

Relationships between BCP mutation and both HBV concentration and Pre-S1 antigen in HBV infected patients

Objective:To investigate the relationships between BCP mutation and both Pre-S1 antigen and HBV DNA concentration in HBV infected patients.Methods:ELISA was applied to detect Pre-S1 antigen, Fluorescence quantitative PCR was used to quantify the HBV DNA concentration, PCR-microplate nucleotide acid hybridization ELISA was used to detect the gene mutation of 1762(A-T) and 1764(G-A) in BCP in 94 HBeAg positive patients respectively.Results:Pre-S1 antigen was positive in 65 of 94 tested samples. BCP gene mutation had taken place in 51 of them and the positive rate was 78.5%. While 23 appeared to be BCP positive in 29 Pre-S1 antigen negative group and the mutation rate was 79.3%. HBV DNA concentrations in gene mutation group were significantly higher than those in BCP negative group.Conclusions:In HBeAg positive patients, the existence of Pre-S1 antigen was in no relation to BCP variation. But BCP variation was directly interrelated to the HBV DNA concentration;both of them can reflect the severity prognosis and of the disease.