| 可变串联重复序列的高度多态性用于骨髓移植植入验证 |
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| 引用本文： | 冯晓勤,钱新华.可变串联重复序列的高度多态性用于骨髓移植植入验证[J].中华医学遗传学杂志,1996,13(1):36-38. |
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| 作者姓名： | 冯晓勤 钱新华 |
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| 作者单位： | 广州南方医院儿科 |
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| 摘 要： | 采用聚合酶链式反应（ＰＣＲ）扩增可变串联重复序列（ｖａｒｉｂａｌｅｎｕｍｂｅｒｔａｎｄｅｍｒｅｐｅａｔ，ＶＮＴＲ）的３３．６位点，加地高辛标记的位点特异性寡核苷酸探针杂交的方法，制作出ＰＣＲ－ＤＮＡ片段长度图像，它具有良好的个体特异性，遗传稳定性和同一性，灵敏度达０．５％。运用该方法成功地对１例慢性粒细胞性白血病患者进行了骨髓移植后的植入验证。
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| 关 键 词： | 可变串联重复序列，聚合酶链反应，地高辛，长度多态性，骨髓移植 |
THE APPLICATION OF HYPERPOLYMORPHIS OF VNTR TO DOCUMENTING MARROW ENGRAFTMENT AFTER BONE MARROW TRANSPLANTION |
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| Feng Xiaoqin, Qian Xinhua, Huang Zhiguang,et al..THE APPLICATION OF HYPERPOLYMORPHIS OF VNTR TO DOCUMENTING MARROW ENGRAFTMENT AFTER BONE MARROW TRANSPLANTION[J].Chinese Journal of Medical Genetics,1996,13(1):36-38. |
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| Authors: | Feng Xiaoqin Qian Xinhua Huang Zhiguang |
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| Institution: | Feng Xiaoqin, Qian Xinhua, Huang Zhiguang, et al. |
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| Abstract: | PCR-DNA fingerprint was completed by amplification of VNTR'S(variable number tandem repeat) locus 33.6 and hybridization with digoxingenin-lableled locus-specific oligonucleotide. It is individually specific,genetically stable and identical. The sensitivity of this method was proved to be 0.5% . One case of CML was successfully documented marrow engraftment after bone marrow transplantation by this way. |
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| Keywords: | Variable number tandem repeat Polymerase chain reaction Digoxingenin Length polymorphis Bone marrow transplantation |
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