褶纹冠蚌的脑、足神经节内Glu、GABA、5-HT、ACh和DA能神经元的分布

目的:观察谷氨酸(glutamate,Glu)、γ-氨基丁酸(γ-aminobutyric acid,GABA)、5-羟色胺(5-hydroxytryptamine,5-HT)、乙酰胆碱(acetylcholine,ACh)和多巴胺(dopamine,DA)神经元在褶纹冠蚌中枢神经系统内的分布。方法:免疫组织化学染色技术和免疫印迹技术。结果:脑神经节外侧胞体层存在大量的GABA能神经元,偶见胞体较大的Glu、5-HT、ACh和DA能神经元,其中央纤维网可见丰富的GABA能神经末稍及少量的Glu、5-HT、ACh和DA能神经末梢。足神经节外侧胞体层亦含有大量的GABA能神经元,可见散在的Glu、5-HT、ACh和DA能神经元,其中央纤维网可见丰富的GABA能神经末稍及零星的Glu、5-HT、ACh和DA能神经末梢。结论:褶纹冠蚌脑和足神经节均含有GABA、Glu、5-HT、ACh和DA能神经元,但这五类神经元的数量和密度有所不同。

Distribution of glutamate-,GABA-,5-HT-,Ach-and DA-containing neurons in the cerebral and pedal ganglia of Cristaria plicata Leach

Objective:To observe the distribution of glutamate(Glu)-,GABA-,serotonin(5-HT)-,acetylcholine(ACh)-and dopamine(DA)-containing neurons in the central nervous system of Cristaria plicata Leach.Methods:Immunohistochemical staining method and Western Blot analysis technique were used.Results:The cellular layer of the cerebral ganglion contained numerous GABAergic neurons and a small number of Glu-,5-HT-,ACh-and DA-containing neurons with relatively big cell body,and the neuropil of the cerebral ganglion has a dense distribution of GABAergic fibers,with scattered nerve endings showing Glu-,5-HT-,ACh-and DA-like immunoreactivities.Similarly,the cellular layer of the pedal ganglion contained abundant GABAergic neurons but scattered Glu-,5-HT-,ACh-and DA-containing neurons,and the neuropil of the pedal ganglion had dense GABAergic fibers and sporadic terminals showing Glu-,5-HT-,ACh-and DA-like immunoreactivities.Conclusion:The present results indicate that the cerebral and pedal ganglia of the Cristaria plicata Leach have Glu-,GABA-,5-HT-,ACh-and DA-containing neurons,but the quantity and density of these neurons are different.