肺癌细胞凋亡中放线菌素D的作用研究

目的:探讨热休克蛋白在Act-D诱导肺癌GLC-82细胞凋亡中的作用,为进一步探讨HSP抗凋亡机制及肺癌生物治疗提供实验依据。方法:体外培养的肺癌GLC-82细胞于RPMI1640、10%胎牛血清、5%CO2孵育,取指数生长期细胞1×105/ml接种反应板24h,分为非热休克组(NHS)和热休克组(HS),两组分别由不同浓度(0,5,10,20μg/m1)放线菌素D(Act-D)诱导,作用4 h。采用脱氧核苷酸末端转移酶介导的dUTP缺口末端标记法(TUNEL)和3H-胸腺嘧啶脱氧核苷(3H-TdR)掺入试验检测肿瘤细胞凋亡指数(AI)及DNA合成情况。结果:HS组比NHS组凋亡指数明显下降(P<0.01);cpm值明显升高(P<0.01),呈现剂量依赖效应。结论:热休克反应对肺癌GLC-82细胞经Act-D诱导凋亡具有抑制作用,此作用与Act-D呈剂量依赖效应。

Study on lung cancer cells apoptosis induced by Act-D

Objective:To investigate the effect on HSP on lung cancer cells GLC-82 apoptosis induced by Actinomycin-D.Methods:Lung cancer cells which were cultured in vitro were divided into NHS and HS groups before treated by Act-D.TUNEL was used to exam apoptosis index and 3H-TdR test is used to examing DNA synthsis.Results:The apoptosis index of HS group significantly decreased than that in NHS group(P<0.01).And the cpm of HS group significant increase than that in NHS group(P<0.01),which were depended on the dosage of Act-D.Conclusion:This paper results indicate that HSP has significant inhibition of apoptosis induced by Act-D in lung cancer cells in vitro.