热带假丝酵母菌对氟康唑耐药性分析 |
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引用本文: | 应颖,刘发娣,熊英,莫冰,卢婷,迟利军,龚甜,阎燕,郭晓,黄孝天.热带假丝酵母菌对氟康唑耐药性分析[J].中国公共卫生,2010,26(4). |
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作者姓名: | 应颖 刘发娣 熊英 莫冰 卢婷 迟利军 龚甜 阎燕 郭晓 黄孝天 |
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作者单位: | 1. 南昌大学医学院微生物学教研室,江西,南昌,330006 2. 江西省儿童医院检验科 3. 江西省疾病预防控制中心疾病控制所 4. 南昌大学第二临床学院2005级 5. 南昌大学第一临床学院2006级 6. 上海交通大学医学院病原生物学教研室 |
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基金项目: | 江西省研究生创新专项资金项目,江西省自然科学基金项目,江西省教育厅科技计划项目 |
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摘 要: | 目的研究热带假丝酵母菌分离株对氟康唑的耐药性,并探讨其耐药的分子机制。方法检测142株热带假丝酵母菌对氟康唑药物的敏感性,PCR扩增热带假丝酵母菌ERG11全基因序列,DNA测序后经生物信息学分析CtERG11基因突变。结果142株热带假丝酵母菌中,128株对氟康唑敏感,占90.1%;4株为氟康唑剂量依赖(S-DD)株,占2.8%;10株对氟康唑耐药,占7.1%;CtERG11序列分析发现,氟康唑敏感株中未见基因突变;S-DD株中出现G1362A和C1404T沉默突变;耐药株中共出现T225C、A242C、T243C、G264A、A395T、C461T和G1362A等7种点突变,并导致D81A、Y132F和S154F氨基酸突变。结论D81A、Y132F和S154F氨基酸突变可能与热带假丝酵母菌对氟康唑耐药有关。
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关 键 词: | 热带假丝酵母菌 CtERG11基因突变 氟康唑 耐药性 |
Mechanism of fluconazole resistance of Candida tropicalis in clinical isolates |
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Abstract: | Objective To investigate fluconazole resistance of Candida tropicalis and its molecular mechanism.MethodsOne hundred and forty-two clinical isolates of Candida tropicalis were collected.Ruconazole susceptibility was tested in vitro with broth microdilution method M27-A2.,The CtERG11 genes of Candida tropicalis WCre amplified and sequenced.Results Of the 142 isolates collected,128(90.1%)were susceptible to fluconazlce,4(2.8%)were susceptible dose-dependent(S-DD)and 10(7.1%)were fluconazole-resistant.The CtERG11 gene sequences of Candida tropicalis isolates were compared with that of Candida tropicalis ATCC 750 and the fluconazole-susceptible strains were completely identical with Candida tropicalis ATCC 750.Two silent mutations of G1362A and C1404T were detected in the fluconzalce S-DD strains;seven nucleotide substitutions of T225C,A242C,T243C,G264A,A395T,C461T and G1362A were found in the four fluconazole-resistant isolates,which resulted in missense mutations of D81A,Y132F and S154F.The nucleotide substitutions of A242C.T243C,G1362A and C1404T were detected and D81A was a novel amino mutation.CondusionThe fluconazole-resistant rate of Candida tropicalis is 7.1%and the missense mutations of D81A,Y132F,and S154F in CtERG11 gene seem to be associated with the fluconazloe resistance of Candida tropicalis. |
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Keywords: | Candida tropicalis CtERG11 gene mutation fluconazole drug resistance |
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