广东省香港海鸥菌感染性腹泻1例分析

目的了解广东省获得性胃肠炎患者中是否存在香港海鸥型菌(LH)感染。方法2009年4月～12月选择江门市各级医院门诊腹泻患者,采集肛拭子标本,培养分离可疑菌株,经革兰染色、生化鉴定、特异性片段PCR扩增和16S rRNA基因序列测定,进行香港海鸥型菌的鉴定。结果在804例腹泻患者标本中,分离并鉴定1株香港海鸥型菌,符合已有报道菌的菌落特点和生化特征,命名为江门株(JM1),16S rRNA目的基因扩增长度为426 bp,基因序列长度为1 360 bp,与香港分离菌株(NR-025167)同源性为99%,确诊为香港海鸥型菌感染病例。结论证实广东省内社区获得性胃肠炎患者中存在香港海鸥型菌的感染。

Laribacter hongkongensis isolated from a patient with community-acquired gastroenteritis in Guangdong province

ObjectiveTo explore Laribacter hongkongensis infection in community-acquired gastroenteritis patients in Guangdong province.MethodsAll fecal sw abs were collected from outpatients with community-acquired diarrhea in selected hospitals.Gram stain,biochemical identification,PCR of specific fragments,and 16S rRNA gene sequencing were employed to identify the bacteria isolated from April to December 2009 in Jiangmen city.ResultsOne case was Laribacter hongkongensis positive among 804 specimens from diarrhea patients,which was in line with the existing coverage of the characteristics and biochemical characterization of bacterial colonies,and named it as JM1.ConclusionThe study proves the presence of Laribacter hongkongensis in the feces of a patient with community-acquired gastroenteritis in Guangdong province.