| 新生大鼠少突胶质前体细胞低糖缺氧损伤模型的建立 |
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| 引用本文： | 姚瑞芹,王兴启,刘轩,于红丽,袁红花,杨丽华. 新生大鼠少突胶质前体细胞低糖缺氧损伤模型的建立[J]. 神经解剖学杂志, 2011, 27(2): 135-139 |
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| 作者姓名： | 姚瑞芹 王兴启 刘轩 于红丽 袁红花 杨丽华 |
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| 作者单位： | 徐州医学院神经生物教研室,徐州,221002 |
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| 基金项目： | 国家自然科学基金，江苏省高校自然科学基金，徐州医学院院长专项基金 |
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| 摘 要： | 目的:利用连二亚硫酸钠(Na2S2O4)建立少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)低糖缺氧损伤模型。方法:取新生1 d Sprague-Dawley(SD)大鼠大脑皮质,体外混合及纯化培养获得OPCs,O4免疫荧光染色鉴定;取纯化2 d的OPCs,分为正常组和Na2S2O4损伤组,Na2S2O4损伤组又分为0.5、1 h和1.5 h三组,倒置显微镜下观察各组细胞形态;CCK-8法检测细胞存活率;透射电镜观察细胞超微结构。结果:免疫荧光染色鉴定显示纯化的细胞绝大部分为O4阳性;形态学观察显示,缺氧0.5 h,大部分细胞突起回缩,胞体变圆,颜色变暗,随低糖缺氧损伤时间的延长OPCs逐渐脱壁,1.5 h时只有少数贴壁细胞;CCK-8法检测显示损伤0.5、1 h和1.5 h组细胞的存活率显著降低,与正常组比较均有统计学意义,损伤后1.5 h与0.5 h比较,细胞的存活率具有显著性差异;电镜观察损伤1.5 h后的细胞,线粒体肿胀,有些细胞胞核固缩成块,细胞器破碎。结论:用浓度为2mmol/L Na2S2O4的低糖培养基处理OPCs 1.5 h可以建立有效的低糖缺氧损伤模型。
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| 关 键 词： | 少突胶质前体细胞 细胞培养 连二亚硫酸钠 低糖 大鼠 |
The establishment of oligodendrocyte precursor cells low glucose and oxygen deprivation model in neonatal rat |
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| Yao Ruiqin,Wang Xingqi,Liu Xuan,Yu Hongli,Yuan Honghua,Yang Lihua. The establishment of oligodendrocyte precursor cells low glucose and oxygen deprivation model in neonatal rat[J]. Chinese Journal of Neuroanatomy, 2011, 27(2): 135-139 |
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| Authors: | Yao Ruiqin Wang Xingqi Liu Xuan Yu Hongli Yuan Honghua Yang Lihua |
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| Affiliation: | Yao Ruiqin,Wang Xingqi,Liu Xuan,Yu Hongli,Yuan Honghua,Yang Lihua(Department of Neurobiology,Xuzhou Medical College,Xuzhou 221002,China) |
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| Abstract: | Objective:This study was design to establish oligodendrocyte precursor cells(OPCs) low glucose and oxygen deprivation model with sodium dithionite(Na2S2O4) in neonatal rat.Methods:The OPCs from cerebral cortex of newborn 1 day Sprague-Dawley(SD) rat were cultured and purfied and identified by immmunofluorescent staining with O4 antibody.The purified OPCs were divided into normal group and model group after being cultured 2 days,and the model group was further divided into 0.5,1 and 1.5 h groups.The morpholo... |
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| Keywords: | oligodendrocyte precursor cells cell culture Na2S2O4 low glucose rat |
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