心房钠尿肽对脂多糖所致大鼠急性肺损伤的影响

目的:探讨心房钠尿肽(ANP)对脂多糖(LPS)血症大鼠急性肺损伤的作用和机制。方法: 大鼠静脉给予LPS(2 mg·kg^－1)后立即静脉给予ANP(2 μg·kg^－1),记录动物平均动脉血压(MAP)、检测血浆一氧化氮(NO)和内皮素(ET)浓度、测定肺水含量并做肺组织病理学检查。结果: 给予LPS的大鼠,MAP持续下降,至4 h MAP(8.1±2.6)kPa;血浆NO和ET浓度均显著升高(P<0.01 vs control);4 h肺湿干比(5.15±0.43),显著高于对照组(P<0.05);大鼠肺组织病理学检查呈现肺间质水肿。LPS＋ANP组大鼠MAP在给药初期的短暂下降后逐步回升,至4h MAP(13.4±2.9)kPa(P<0.05 vs LPS);4 h血浆NO水平和ET浓度均显著下降(P<0.05 vs LPS),但仍明显高于对照组(P<0.01 vs control);肺湿干比(4.57±0.35)与对照组没有显著差异;肺组织病理学改变较LPS组明显减轻。结论: ANP对LPS引起的急性肺损伤有治疗作用,能调节LPS引起的动脉血压的持续下降,上述作用可能与ANP拮抗ET的产生、降低NO的分泌有关。

Effect of atrial natriuretic peptide on acute lung injury induced by lipopolysaccharide

AIM: To investigate effect of atrial natriuretic peptide (ANP) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rat. METHODS: Mean arterial blood pressure (MAP) was recorded with model 6280 physiology intelligentialize grapher, nitric oxide (NO) and endothelin (ET) concentrations in plasma were measured after lipopolysaccharide (LPS) or following LPS ,ANP was injected into vein in rats. After experiment,lung water as well as pulmonary histopathological changes was measured and observed, respectively. RESULTS: Administration of LPS elicited a persistence decrease in MAP (8.1 kPa±2.6 kPa,at 4 h,P<0.01 vs control); NO and ET concentration in plasma was evident higher than that in control group, respectively (P<0.01); Wet-dry ratio of lung was higher than that in control group (5.15±0.43,at 4 h) (P<0.05); Alveolus detelectasis was observed and pulmonary mesenchyme was thicker than that in control group. No erythrocyte and leukocytes in alveolus,which show an interstitial pulmonary edema, was observed in LPS+ANP group, ANP maintained MAP at higher levels (13.35 kPa±2.93 kPa, at 4 h, P<0.05 vs LPS) after an transient decline when LPS was injected; NO and ET concentration of plasma had all significantly decrease, respectively (P<0.05 vs LPS, at 4 h); Wet-dry ratio of lung was lower than LPS group (4.57±0.35, P<0.05). Compared with control group the ratio was not evident difference (P>0.05); The histopathological of lung displayed markedly improved. CONCLUSION: ANP attenuates ALI induced by LPS in the rat. The effect of ANP may be via decreasing secretion of ET,NO and regulation arterial blood pressure.