川芎嗪对肝缺血再灌注损伤大鼠P-选择素表达的影响

[目的]观察川芎嗪干预肝缺血再灌注损伤大鼠P-选择素表达,探索可能的病理机制,为预防治疗肝细胞缺血再灌注损伤提供依据.[方法]将64只SD大鼠随机分为4组,肉眼观察肝脏形态变化,光镜、电镜观察肝细胞形态变化,免疫组化SABC法检测肝组织中P-选择素表达.[结果]缺血再灌注组可见肝细胞水肿明显,伴空泡形成,间质充血水肿及炎性细胞浸润,再灌注6 h可见肝组织出现明显的点状坏死灶,并可见到灶性坏死;再灌注24 h肝组织可见多发性灶性坏死及片状坏死灶.川芎嗪干预组各时间点肝组织外观与正常肝相似,光镜下肝细胞无明显肿胀,有极少量空泡形成,但无变性或坏死,且间质无明显变化.免疫组化SABC法检测显示,缺血再灌注早期P-选择素即在肝组织中表达,与川芎嗪干预组及假手术组比较,差异有统计学意义(P＜0.01).缺血再灌注组P-选择素表达以1 h时最为明显,与再灌6 h及24 h相比,差异有统计学意义(P＜0.01).[结论]川芎嗪能有效抑制P-选择素的表达,减轻肝缺血再灌注损伤.P-选择素主要在缺血再灌注损伤早期表达.P-选择素介导中性粒细胞滚动黏附,可能是肝缺血再灌注损伤的主要机制之一.

Effects of ligustrazine on P-selectin expression of rat liver with ischemia-reperfusion injury and construction of liver cell

[Objective] To observe the pathologic changes of liver cell in ischemia-reperfusion injury rat under the microscope and electron microscope,and explore the pathologic mechanism of liver ischemia-reperfusion and the effects of ligustrazine.[Methods] Sixty-four Sprague-Danley(SD) rats were devided into four groups.Morphology of liver,hepatic tissue and expression of P-selectin in various groups were observed.[Results] In the hapetic ischemia-reperfusion group,dropsy of liver tissue was obvious and vacuole came into being.Histological damage in point was found after repergusion 6 h and more serious after reperfusion 24 h.These changes were markedly alleviated in the group of ligustrazine treatmemt.P-selectin expressed in early ischemia-reperfusion injury period,which had significant difference with ligustrazine treated group and pseudooperation group(P<0.01),and expressed much obviously at ischemia-reperfusion 60 min,which was more obviously than that at 6 h and 24 h(P<0.01).[Conclusion]P-selectin expressed in early ischemia-reperfusion injury period.Ligustrazine could restrict the expression of P-selectin and reduce the hepatic injury caused by the ischemia-reperfusion.P-selectin mediated neutrophil rolling and adherence,which might contribute to liver ischemia-reperfusion injury.