Identification of cyclophilin A from human decidual and placental tissue in the first trimester of pregnancy |
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| Authors: | Meier, Ursula Beier-Hellwig, Karin Klug, Jorg Linder, Dietmar Beier, Henning M. |
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| Affiliation: | 1Department of Anatomy and Reproductive Biology, Medical Faculty RWTH University of Aachen, D-52057 Aachen 2Institute of Molecular Biology and Tumor Research Philipps University Marburg, D-35037 Marburg 3Department of Biochemistry Justus Liebig University, D-35392 Giessen, Germany |
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| Abstract: | The protein patterns of tissue homogenates from human deciduaand placenta of first trimester pregnancies were investigated.Particular attention was paid to the low molecular weight componentsof these tissues, since substantial evidence has accumulatedthat some of these smaller proteins show a characteristic cyclicand pregnancy expression. Two specific bands were purified fromhomogenates of first trimester decidua and placenta using gelfiltration and anion exchange chromatography. These bands wereseparated by gel electrophoresis and blotting onto polyvinylidendifluoridemembrane. Partial amino acid sequencing of both proteins revealedsequences identical to human cyclophilin A. One protein wassequenced V-N-P-T-V-F-F-D-I-A-V-D-G-E-P-L-G-R-(X)-S-F-E-L-F-A-D-K-V-Pand identified as the 17 kDa isoform of cyclophilin A. The otherprotein was sequenced V-N-P-T-V-F-F-D-I-A and identified asthe 18 kDa isoform of cyclophilin A. cyclophilin A/decidua/placenta/progesterone/progesterone receptor |
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