延迟整流性K+通道在人肝癌细胞增殖中的作用

目的：研究延迟整流性Ｋ＋通道在人肝癌细胞增殖中的作用。方法：采用膜片钳技术的全细胞记录方式记录人肝癌细胞在不同浓度ＥＧＦ中延迟整流性Ｋ＋通道的跨膜电流。利用氚标记的胸腺嘧啶核苷（３ＨＴｄＲ）掺入技术观察ＥＧＦ和Ｋ＋通道阻断剂（ＴＥＡ）对人肝癌细胞ＤＮＡ合成的影响。结果：发现表皮生长因子（ＥＧＦ）促进肝癌细胞增殖的同时使肝癌细胞膜离子通道的跨膜Ｋ＋电流增加。Ｋ＋通道阻断剂四乙胺（ＴＥＡ）能显著抑制ＥＧＦ促进ＤＮＡ合成的作用，具有明显的量效关系和时间依赖性，但对细胞的活力无明显影响。结论：人肝癌细胞的增殖活动需要延迟整流性Ｋ＋通道参与。

The role of delayed rectifier K+ channel in the proliferation of the human hepatocyte carcinoma cells

Objective: To study the role of the delayed rectifier K + channel in the proliferation of the human hepatocyte carcinoma cells. Methods: The transmembrane current of human hepatocyte carcinoma cells was recorded by the whole cell recording technique of patch clamp in different concentration of EGF. The effect of EGF and K + channel blocker on the DNA synthesis of human hepatic carcinoma cells was observed by 3H TdR incorporation technique. Results: EGF promoted the cell proliferatetion of the human hepatocyte carcinoma cells. At the same time, EGF made the K + channel current of hepatocyte carcinoma cells increased. TEA of K + channel blocker could significantly inhibited DNA synthesis induced by EGF. There was a linear relationship between the concentration of TEA and the effect of inhibition as well as time dependence. But TEA didn't reduce the viability of hepatocyte carcinoma cells. Conclusion: K + channel was necessary for the proliferation of the human hepatocyte carcinoma cells.