| Toll样受体介导H37Rv诱导的单核细胞趋化蛋白-1表达 |
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| 引用本文： | 李敏超,杜先智,周向东.Toll样受体介导H37Rv诱导的单核细胞趋化蛋白-1表达[J].第三军医大学学报,2010,32(20). |
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| 作者姓名： | 李敏超 杜先智 周向东 |
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| 作者单位： | 重庆医科大学附属第二医院呼吸内科,重庆,400010 |
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| 基金项目： | 国家自然科学基金(30872261); 重庆市卫生局资助项目(2007)~~ |
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| 摘 要： | 目的探讨Toll样受体2(TLR2)、TLR4介导的免疫调节信号通路在结核分枝杆菌感染诱导的单核细胞趋化蛋白-1(monocyte chemo attractant protein-1,MCP-1)产生中所起的作用。方法建立人型结核分枝杆菌H37Rv感染小鼠模型,获取模型组及对照组小鼠支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及肺泡巨噬细胞(alveolar macro-phage,AM),对BALF中AM进行计数,采用酶联免疫吸附试验(ELISA)法测定BALF中MCP-1水平,Western免疫蛋白印迹(Western blot)方法检测肺组织中MCP-1蛋白含量,逆转录聚合酶链反应(RT-PCR)测定模型组及对照组AM中TLR2、TLR4mRNA的表达;抗体干预组的AM用TLR2、TLR4抗体预处理并与H37Rv菌株共培养,并设未予以抗体预处理的PBS组及未作任何处理的对照组,分别用ELISA、Western blot法检测AM培养液中及AM中的MCP-1水平,以观察TLR2、TLR4在H37Rv诱导MCP-1生成所起的作用。结果模型组BALF中AM计数及MC...
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| 关 键 词： | 结核 肺泡巨噬细胞 Toll样受体 MCP-1 |
Toll-like receptor mediates MCP-1 expression induced by mycobacterium tuberculosis H37Rv |
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| Li Minchao,Du Xianzhi,Zhou Xiangdong.Toll-like receptor mediates MCP-1 expression induced by mycobacterium tuberculosis H37Rv[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(20). |
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| Authors: | Li Minchao Du Xianzhi Zhou Xiangdong |
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| Institution: | Li Minchao,Du Xianzhi,Zhou Xiangdong(Department of Respiratory Medicine,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,China) |
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| Abstract: | Objective To explore the role of Toll-like receptor(TLR)2-and TLR4-mediated immune regulating signal pathway on the production of monocyte chemo attractant protein-1(MCP-1)induced by infection of mycobacterium tuberculosis.MethodsMouse model of H37Rv infection was made by injecting H37Rv through tail vein.Alveolar macrophage(AMs)and bronchoalveolar lavage fluid(BALF)were obtained from H37Rv group and control group.The numbers of AM in BALF were counted.MCP-1 protein levels in BALF and lung tissue were deter... |
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| Keywords: | tuberculosis alveolar macrophage Toll like receptors monocyte chemo attractant protein-1 |
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