石榴叶鞣质对高血脂高血糖模型动物脂代谢的影响

目的 :观察石榴叶鞣质对高脂模型鼠脂代谢的影响 ,以及体外对HMG CoA还原酶的抑制作用。方法 :造大鼠高脂模型 ,以四氧嘧啶诱导高血糖伴高血脂小鼠模型 ,检测TC、TG等生化指标 ;制备大鼠肝微粒体HMG CoA还原酶液 ,测 1min内A34 0nm的变化。结果 :石榴叶鞣质大中剂量 (15 0、75mg kg)对高脂大鼠的TC和TG具有显著的降低作用 ,大小剂量 (30 0、75mg kg)对高血糖伴高血脂小鼠的TC降低作用明显 ,体外抑制HMG CoA还原酶的最低有效浓度为 1× 10 - 6 g mL ,抑制率 15 16 %。结论 :石榴叶鞣质对高脂模型鼠脂代谢紊乱具有较好的调节作用 ,体外可较强的抑制HMG CoA还原酶活性。

The Effect of Pomegranate Leaf Tannide on Hyperlipidemic Models

Objective:To explore the effect of pomegranate leaf tannide (PLT) on lipid metabolite of high-lipid rats and its inhibition on HMG-CoA reductasein vitro.Methods:The high-lipid rat model and high-lipid wiih high-sugar mice model induced by intraperitoneal injection of alloxan were used.The contents of TC and TG of both models was measured.Rat liver microsome HMG-CoA reductase was prepare to measure the content changes of A₃₄₀in 1minute.Results:PLT can remarkably decrease TC and TG of high-lipid rat at doses of 150 and 75 mg/kg,and its reducing effect on TC of high-lipid with high-sugar mice at doses of 300 and 75mg/kg is also obvious.The minimal inhibition concentration of HMG-CoA reductasein vitrois 1×10^-6g/mL with inhibition percentage of 15.16%.Conclusion: PLT has a noticeable activity of modulating lipid metabolite of high-lipid rat and inhibiting HMG-CoA redutasein vitro.