玉郎伞皂苷对四氯化碳诱导大鼠肝纤维化的影响

目的研究玉郎伞皂苷(YLSS)对四氯化碳(carbontetrachloride,CCl4)所致大鼠肝纤维化的药理作用,并探讨其作用机制。方法将SD大鼠随机分成模型组及空白对照组(NC),模型组大鼠以50%CCl4食用油溶液为诱导剂灌胃造模,NC组灌胃给予食用油。将病理检查确认形成肝纤维化的SD大鼠,随机分成模型对照组(MC)和药物干预组。药物干预组分为4小组,分别灌胃给予YLSS(20、40和80mg.kg-1)及秋水仙碱片(0.20 mg.kg-1),模型组给予等剂量NS,连续给药4周。末次给药24 h后处死大鼠,采集血清及肝组织,检测大鼠血清中AST、ALT活性,测定肝组织中SOD、MDA、GSH和GSH-Px的含量,并观察肝组织病理学改变。结果各剂量YLSS和阳性药能降低大鼠血清中CCl4所致异常升高的AST、ALT水平(P<0.01),提高肝组织SOD、GSH含量(P<0.01),并降低异常升高的MDA含量(P<0.05或P<0.01);各剂量YLSS能升高大鼠肝组织GSH-Px(P<0.01);中、高剂量YLSS及阳性药能够减轻肝细胞损伤程度(P<0.01)。结论玉郎伞皂苷对CCl4诱导的大鼠肝纤维化具有一定的抑制作用,其机制可能与其清除自由基、抑制脂质过氧化有关。

The Effect of yulangsan saponins on liver fibrosis induced by carbon tetrachloride in rats

Aim To investigate the effect of yulangsan saponins(YLSS) on CCl4-induced liver fibrosis in rats and its mechanism.Methods The SD rats were divided into two groups randomly: hepatic fibrosis group and normal control group.The rats of hepatic fibrosis group were induced by intragastric administration(i.g.) of 50% CCl4,and the normal control group were given normal saline(NS).The rats of hepatic fibrosis group confirmed by the pathological inspection were divided into 2 subgroups randomly: drug intervention group and the model group which were treated with NS.The drug intervention group were divided randomly into 4 subgroups: 3 different doses(20 mg·kg-1,40 mg·kg-1 and 80 mg·kg-1) of YLSS groups and a positive control group(colchicine tablets 0.20 mg·kg-1).All rats were treated with drugs of NS for 4 consecutive weeks by i.g.24 hours after the last administration of drugs,all rats were sacrificed,and the blood serum and hepatic tissue were taken quickly.The activities of ALT,AST in the serum and the expressions of SOD,MDA,GSH,GSH-Px in the hepatic tissue were analyzed,and the degree of hepatic injury was examined.Results Compared with the model control group,in all doses of YLSS groups and positive control group,the activities of AST and ALT in serum were decreased significantly(P<0.01).The levels of SOD and GSH in liver were increased significantly(P<0.01),but MDA was increased significantly in liver(P<0.05 or P<0.01).Meanwhile,GSH-Px activity was increased in all doses of YLSS groups(P<0.01).And the degree of hepatic injury in high dose and middle dose YLSS groups and positive control group could be lessened(P<0.05 or P<0.01).Conclusion YLSS has certain curative effect on CCl4-induced liver fibrosis in rats.Its mechanism may involve attenuating free radical and inhibiting the lipid peroxidation.