siRNA沉默Twist基因对鼻咽癌细胞CNE-2增殖的影响

目的探讨RNA干扰沉默Twist基因对人鼻咽癌细胞株CNE-2的迁移、侵袭和增殖等生物学活性的影响。方法构建Twist-shRNA表达载体,脂质体法将重组质粒分别转染入鼻咽癌细胞株CNE-2中,RT-PCR法检测Twist mRNA、Western blot法检测Twist蛋白表达量的变化。MTT法检测细胞的增殖,细胞创伤愈合试验观察细胞迁移能力,Tran-swell小室试验检测细胞侵袭能力的变化。结果沉默Twist基因可减慢CNE-2细胞生长速度(与对照组比较P<0.05),降低CNE-2细胞的迁移速度,抑制CNE-2的体外侵袭能力。结论靶向封闭Twist基因的表达,可明显抑制CNE-2细胞的增殖、迁移及侵袭能力,提示Twist可作为鼻咽癌防治的一个新的靶点。

Antitumoral effects of silencing Twist on nasopharyngeal carcinoma cell line CNE-2

Aim To investigate the effects of silencing Twist expression by RNAi on tumor activity in nasopharyngeal carcinoma CNE-2 cells.Methods The TwistshRNA expression vectors were constructed and transfected into CNE-2 cells with lipofectamineTM2000.The mRNA levels of Twist were assayed by RT-PCR.The protein levels of Twist were measured by Western blot.The cell proliferation was determinated by MTT.The migration of cells was detected by cell wound healing trial.The invasion ability of cells in vitro was observed by Matrigel invasion assay.Results The mRNA and protien levels of Twist were decreased significantly by Twist-siRNA.Twist-siRNA effectively inhibited the cell proliferation.The ability of invasion and migration in vitro cell were descended observably by RNAi(P < 0.05).Conclusions Silencing Twist expression by RNAi significantly inhibits the ability of CNE-2 cell proliferation,invasion and migration,Twist may be a potential therapeutic target gene for nasopharyngeal carcinoma.